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Detection of bovine respiratory syncytial virus by RT-PCR |
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| Authors: | Glotov A G Glotova T I Stroganova I Ia |
| Abstract: | The paper presents the results of studying the diagnostic efficiency of RT-PCR for the detection of respiratory syncytial virus in cattle of different ages. Glycoprotein F gene sequences were used as a target for amplification. The sensitivity of the reaction was 10 TCD50/ml and the virus detection rate in biomaterials averaged 19%. samples. That in RT-PCR correlated with the presence of clinical signs in sick animals. |
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