浒苔多糖对2型糖尿病大鼠脂代谢及脂联素mRNA表达影响

目的研究浒苔多糖(Enteromorpha’s polysaccharide,EP)对2型糖尿病大鼠脂代谢影响。方法高糖高脂饲料喂养,结合注射小剂量链脲佐菌素(STZ)建立2型糖尿病大鼠模型,EP采用高(600mg/kg.bw)、中(300mg/kg.bw)、低(150mg/kg.bw)剂量进行干预。测定血脂水平、抗氧化指标;HE染色观察肝脏和胰腺组织结构;RT-PCR测定脂联素(adiponectin,APN)mRNA的表达水平。结果模型组精神萎靡,脱发、多饮、多食、多尿和体重减轻等T2DM症状明显,EP高、中、低剂量组2型糖尿病症状有所减轻。与空白组比较,模型组TC、TG、HDL-C和LDL-C明显较高(P<0.01)。与模型组比较,EP高、中、低剂量组TG明显较低(P<0.01),EP高、中剂量组TC、LDL-C明显较低(P<0.01),EP高、中剂量组HDL-C/TC比值较高(P<0.01)。EP高、中、低剂量组MDA明显较低(P<0.01),SOD较高(P<0.05),EP高、中剂量组GSH-Px较高(P<0.01,P<0.05),EP高、中剂量组脂肪APNmRNA表达较高(P<0.05),EP高、中、低剂量组肝脏细胞脂肪变性程度较轻,胰腺的病理损害较小。结论 EP对2型糖尿病大鼠的脂代谢具有一定的调节作用。[营养学报,2013,35(2):181-185]

EFFECTS OF ENTEROMORPHA'S POLYSACCHARIDE ON LIPID METABOLISM AND ADIPONECTIN GENE EXPRESSION IN TYPE 2 DIABETIC RATS

Objective To study the effect of Enteromorpha's polysaccharide(EP) on lipid metabolism in type 2 diabetes.Methods A model of type 2 diabetes in rats was established by feeding high sugar and fat diet and an injection of STZ.The rats were then given po daily 150,300 or 600 mg/(kg bw) EP for 4 w.After 4w,serum TC,TG,HDL,LDL-C,MDA levels,SOD and GSH-Px activities were measured.The pathological changes of liver and pancreas were also observed by HE staining and the mRNA level of adiponectin(APN) in adipose tissue was detected by real time-PCR.Results The diabetic symptoms such as listlessness,hypotrichosis,polydypsia,polyphagia,polyuria,emaciation in EP groups were lighter than those in model group.Compared with the control group,the levels of TC,TG,HDL and in model group were significantly increased(P<0.01).Compared with the model group,the levels of TG and MDA were significantly reduced(P<0.01) in all EP groups;TC,LDL-C significantly reduced and HDL-C/TC increased in high and middle dose EP groups(P<0.01).SOD activities were increased in high dose EP group(P<0.05).GSH-Px activities and mRNA level of APN were increased(P<0.05) in high and middle dose EP groups.The steatosis of hepatic cells and the pancreatic pathological damage were lighter in all EP groups.Conclusion EP can regulate lipid metabolism in type 2 diabetes.[ACTA NUTRIMENTA SINICA,2013,35(2):181-185]