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| 川麦冬中麦冬皂苷D对照品的制备 | |
| 引用本文： | 卢克刚,张红霞,赵云龙. 川麦冬中麦冬皂苷D对照品的制备[J]. 中国当代医药, 2009, 16(20): 110-112 |
| 作者姓名： | 卢克刚 张红霞 赵云龙 |
| 作者单位： | 1. 济南铁道职业技术学院生物工程系,山东济南,250013 2. 济南京鲁生物技术研发中心,山东济南,250013 3. 山东健康药业有限公司,山东济南,250022 |
| 摘 要： | 目的：建立从麦冬中制备麦冬皂苷D对照品的方法。方法：用萃取、硅胶柱层析、凝胶柱层析、重结晶等方法对麦冬乙醇提取物进行分离纯化，通过MS、IR,^1H—NMR．^13C—NMR进行结构鉴定。结果：薄层色谱法（TLC）、高效液相色谱法（HPLC—ELSD）检测分析，麦冬皂苷D的纯度为99％以上。结论：本方法简便，产品纯度高，该对照品可作为川麦冬及其制剂质量的指标成分。 |
| 关 键 词： | 麦冬 麦冬皂苷D 对照品 |
Preparation of reference substance of ophiopogonin D from tuber roots of Ophiopogon japonicus |
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| LU Kegang,ZHANG Hongxia,ZHAO Yunlong. Preparation of reference substance of ophiopogonin D from tuber roots of Ophiopogon japonicus[J]. http://www.botanicus.org/, 2009, 16(20): 110-112 | |
| Authors: | LU Kegang ZHANG Hongxia ZHAO Yunlong |
| Affiliation: | LU Kegang,ZHANG Hongxia, ZHAO Yunlong (1.Bioengineering Department, Jinan Railway Institute of Technology, Jinan 250013, China; 2.The R＆D Center of Jinglu Bio-technology of Jinan,Jinan 250013,China;3. Shandong Health Pharmacy Co.,Ltd,Jinan 250022,China) |
| Abstract: | Objective：To establish a method for preparing the reference substance of ophiopogonin D from the tuber roots of Ophiopogon japonicus, nethods：Ophiopogonin D was isolated and purified from the ethanol-extract of the tuber roots of Ophiopogon japonicus by solvent extraction, silica gel sephadex LH-20 column chromatography, and crystallization. Its tructure was determined by spectroscopic methods （MS, IR, ^1H- and ^13C-NMR）. The purity of the reference substance was identified with TLC and HPLC-ELSD. Results： TLC and HPLC analysis showed that the purity of ophiopogonin D was not less than 99%. Conclusion： The method developed is simple and effective for producing product with high purity. The product obtained can be used as a reference substance in the quality control and the research of herbal medicine. |
| Keywords: | Tuber roots of Ophiopogon japonicus Ophiopogonin D Reference substance |
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