| 窝洞清洁剂暴露的牙本质中TGF—β超微结构定位 |
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| 引用本文： | 赵守亮,Sloan AJ,Murray PE,Lumely PJ,Smith AJ. 窝洞清洁剂暴露的牙本质中TGF—β超微结构定位[J]. 牙体牙髓牙周病学杂志, 2001, 11(1): 20-23 |
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| 作者姓名： | 赵守亮 Sloan AJ Murray PE Lumely PJ Smith AJ |
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| 作者单位： | 1. 第四军医大学口腔医学院;
2. 英国伯明瀚大学牙科学院, |
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| 摘 要： | 目的：了解不同窝洞清洁剂暴露窗洞内牙本质中TGF－β的能力。方法：采用金标记免疫组化染色,扫描电镜观察。结果：170g/L的EDTA处理组牙本质中有大量TGF－β1暴露,表面玷污层去除彻底;30g/L次的人和100g/L柠檬酸处理组有一定量的TGF－β1暴露,玷污层被部分去除;而PBS且只有少量TGF－β1被暴露。TGF－β2、β3在各组中均未测得。结论：TGF－β1是牙本质中最主要的TGF－β异构体,EDTA是所测试剂中暴露TGF－β最有效的窝清洁剂。
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| 关 键 词： | 窝洞清洁剂 EDTA 次氯酸钠 柠檬酸 牙本质 TGF－β 组织修复 |
| 文章编号： | 1005-2593(2001)01-0020-04 |
| 修稿时间： | 2000-06-25 |
Ultrastructurallocalization of TGF- |
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| ZHAO Shou-liang ,Sloan AJ,Murray PE,et al. Ultrastructurallocalization of TGF-[J]. Chinese Journal of Conservative Dentistry, 2001, 11(1): 20-23 |
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| Authors: | ZHAO Shou-liang Sloan AJ Murray PE et al |
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| Affiliation: | ZHAO Shou-liang *,Sloan AJ,Murray PE,et al |
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| Abstract: | AIM: To investigate the ability of cavity conditioning agents toexpose TGF-βs from mature human dentine at sites of cavity preparation. METHODS: Gold-labelled Immunohistochemical staining and SEM. RESULTS: 170g/L EDTA exposed large amounts of TGF-β1 from dentine and clearly removed any Smear layer present; 30g/L sodium hypochlorite and 100g/L citric acid caused some exposure of TGF-β1 and partial removal of the smear layer, while in PBS treated samples only minimal TGF-β1 was exposed. TGF-β2 and TGF-β3 were not detected in all groups. CONCLUSION: TGF-β1 is the most prevalent TGF-β isoform in dentine and EDTA is the most effective cavity-conditioning agent in exposing this growth factor from dentine. |
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