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Additive effects of earthworm extract with HpD-laser on the enhancement of reactive oxygen production and the inhibition of[~3H]—thymidine.uptake in mouse ascitic tumor cells
作者姓名:孙淑芬  莫简  郭正仁  王多宁  田琼  刘健  李之源
作者单位:Departments of Chemistry Fourth Military Medical University Xi'an 710032,Departments of Chemistry Fourth Military Medical University,Xi'an 710032,Departments of Chemistry Fourth Military Medical University,Xi'an 710032,Departments of Chemistry Fourth Military Medical University,Xi'an 710032,Medical Radiology,Fourth Military Medical University,Xi'an 710032,Central Laboratory,Fourth Military Medical University,Xi'an 710032,Editorial Department of J Fourth Milit Med Univ,Fourth Military Medical University,Xi'an 710032
基金项目:Supported by the National Natural Science Foundation of China No.3860835
摘    要:The effects of combined use of earthworm extract(912)and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S_(180) tumor cells were studied throughchemiluminescence measurement and[~3H]-TdR incorporation assay.The results showed that as com-pared with the control,the intensity of chemiluminescence emitted by tumor cells treatedsimultaneously with 912 and HpD-laser was enhanced more than ten-folds,while that treated with912 or HpD-laser alone was increased only 2~4 folds.The[~3H]-TdR incorporation into tumorcells of the former group was inhibited upto 74.1%,and that of the latter groups decreased onlyby 42.2% and 40.0%,respectively.In accordance with these biochemical changes,the ultrastructuraldamage of tumor cells of the former,combinedly treated group appeared to be the most serious.This suggests an additive effect of 912 with HpD-laser on tumor cells.In addition,if free radicalscavengers,such as catalase and superoxide dismutase,were added to the reaction systembefore chemiluminescence assay,the luminescent enhancement effect mentioned above was dramaticallyalleviated,implying the presence of O_2~ and H_2O_2 in the system.Therefore,as to the toxic effecton tumor cells,912 and HpD-laser are not only additive in efficiency,but also similar in theunderlying mechanism of action.

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