| 对羟苯甲酸3-羟化酶初步纯化及进化起源探讨 |
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| 引用本文： | 陈瑞,细川桂一.对羟苯甲酸3-羟化酶初步纯化及进化起源探讨[J].首都医学院学报,2002,23(2):101-104. |
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| 作者姓名： | 陈瑞 细川桂一 |
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| 作者单位： | 首都医科大学生物化学教研室(陈瑞
)，日本食品综合研究所微生物机能工学研究室(细川桂一) |
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| 基金项目： | 日本国两备圣园纪念财团助成金资助项目 |
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| 摘 要： | 经超声破碎、硫酸铵分级沉淀、凝胶过滤、离子交换层析和SDS PAGE ,从Comamonastestosteroni菌株中获得了相对分子质量为 4 .5万的对羟苯甲酸 3 羟化酶 (POB3 HOase) ;测定了其N 末端 2 1个氨基酸序列 ,并与文献报道来源于不同菌株的POB3 HOase序列进行了同源性比较 ,结果提示它们具有相同的进化起源。
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| 关 键 词： | 对羟苯甲酸3-羟化酶 纯化 进化起源 丛毛单孢菌 |
| 收稿时间： | 2001-11-20 |
| 修稿时间： | 2001年11月20 |
Preliminary Purification and Pursuit of Evolutionary Origin of P-hydroxybenzoate 3-hydroxylase from Comamonas Testosteroni |
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| Chen Rui.Preliminary Purification and Pursuit of Evolutionary Origin of P-hydroxybenzoate 3-hydroxylase from Comamonas Testosteroni[J].Journal of Capital University of Medical Sciences,2002,23(2):101-104. |
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| Authors: | Chen Rui |
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| Institution: | Chen Rui Department of Biochemistry,Capital University of Medical Sciences Hosokawa Keiichi National Food Research Institute,Ministry of Agriculture Forestry and Fisheries |
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| Abstract: | The P-hydroxybenzoate 3-hydroxylase (POB-HOase) with 45 thousand relative molecular mass from Comamonas testosteroni was purified by using sonic crushing, ammonium sulfate fractionation, molecular sieve chromatography, exchange chromatography and SDS-PAGE. The N-termina lamino acid sequence was determined up to 21 residues. The amino acid sequence was compared with the known N-terminal sequences of POB3-HOases from Pseudomonas aeruginosa, Pseudomonas fluorescens and Acinetobacter calcoaceticus. The high degree of homology observed between the POB3-HOases from Comamonas and those of Pseudomonas and Acinetobacter indicates the common evolutionary origin of POB3-HOase among these soil bacteria of different genera. |
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| Keywords: | para-hydroxybenzoare 3-hydroxylase purification evolutionary origin Comamonas testosteroni |
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