重组sCR1对大鼠急性脊髓损伤组织补体表达的影响

目的探讨重组可溶性补体受体Ⅰ型(sCR1)对大鼠急性脊髓损伤组织补体表达的影响及对脊髓损伤的保护作用.方法采用改良Allen重物打击法制成SD大鼠脊髓急性损伤模型,采用斜板实验评定sCR1组与生理盐水(NS)组大鼠下肢运动神经功能,观察两组在伤后12h,1,3,7,14 d时间点损伤组织C3c,C9及CD59阳性表达的部位及时程,并比较组间差异.结果sCR1组在伤后3,7,14d时间点大鼠下肢运动功能明显优于NS组(分别为P＜0.05,P＜0.05,P＜0.01);sCR1组在伤后各个时间点C3c,C9阳性表达均明显轻于NS组,差异有非常显著性(P＜0.01);sCR1组在伤后12h,1,3 d时间点CD59的表达明显轻于NS组,差异有非常显著性(P＜0.01),伤后7d两组间差异有显著性(P＜0.05),伤后14 d两组间差异无显著性.结论重组可溶性补体受体Ⅰ型可显著减轻急性脊髓损伤组织中补体的表达,可通过抑制补体系统激活机制减轻继发性脊髓损伤.

Effects of recombinant sCR1 on complement in acute spinal cord injury tissue of rats

To discuss the effects of recombinant soluble alexin receptor type I (sCR1) on complement expression in acute spinal cord injury tissue of rats and the protection of it on spinal cord injury. SD rat models by modified Allen's assay for acute spinal cord injury were adopted. Motor nerve function of rat lower extremities in sCR1 group and normal saline (NS) group were evaluated by the tiltboard experiment. C3c, C9 and CD59 positive expression sites and time phase in injury tissue at 12 h, 1, 3, 7 and 14 d after injury in the two groups were observed and the differences between the two groups were noticed. Rat motor function in sCR1 group at 3, 7 and 14 d was obviously better than that in NS group (P <0.05, P <0.05, P <0.01); C3c and C9 positive expression in sCR1 group at each time point after injury was obviously less than that in NS group with highly significant difference (P <0.01); CD59 positive expression in sCR1 group was obviously less than that in NS group with highly significant difference (P <0.01) at 12 h, 1 and 3 d, with significant difference (P <0.05) at 7 d after injury, with no significant difference at 14 d after injury. [Conclusions] Recombinant soluble complement receptor type I (sCR1) can obviously lessen the complement expression in acute spinal cord injury tissue. It can relieve secondary spinal cord injury by inhibiting the activation of the complement system.