实验性肝纤维化大鼠层粘连蛋白含量的变化

用四氯化碳诱导大鼠肝脏纤维化，采用酶联免疫吸附法（ＥＬＩＳＡ）检测大鼠血清和肝组织匀浆中层粘连蛋白（ＬＮ）含量，并分析ＬＮ与肝功能及肝组织学病理改变之间的关系。结果发现，血清ＬＮ在大鼠肝脏受损伤后早期即出现增加，并随肝组织损害程度的加重而递增，与肝功能改变及病理损伤有一定相关性。肝匀浆中ＬＮ含量在肝损伤晚期才升高，与肝功能指标没有相关性，提示，血清ＬＮ含量改变是反映肝脏损伤的敏感指标，可用于动态观

Changes of Laminin Contents in Carbon Tetrachloride Induced Fibrotic Rat Liver

iver fibrosis was induced in rats with carbon tetrachloride. Assays were performed usingELISA method on laminin(LN)in both serum and liver homogenate. The results demonstrated asignificant increase in the serum levels of LN that occured early and in the late stage of liver fibro-sis.The serum concentrations of LN in different stages paralleled the progressed liver damage andthe liver function tests.In contrast,no significant increase was seen in the liver homogenate levelof LN in the early stage of liver fibrosis,but only in the late stage. The results suggested that im-munoassay for the LN in serum is a more sensitive index for liver damage and may be behelpful tothe diagnosis of liver fibrosis.