|
|||||
|
|
| 应用MTT法进行体外抗肿瘤药敏试验影响因素研究 | |
| 引用本文： | 魏文青,赵满仓,刘晶,焦娟,张艳,付瑶,安平. 应用MTT法进行体外抗肿瘤药敏试验影响因素研究[J]. 华北国防医药, 2008, 20(6) |
| 作者姓名： | 魏文青 赵满仓 刘晶 焦娟 张艳 付瑶 安平 |
| 作者单位： | 北京军区总医院中心实验科; |
| 摘 要： | 目的:探讨应用MTT法进行体外抗肿瘤药敏试验的影响因素,旨在建屯一种MTT法抗肿瘤药敏试验的规范化流程.方法:MTT法测定不同浓度人外周血单个核细胞(PBMC)对不同浓度的奥沙利铂与紫杉醇的敏感性,同时优化选择培养时间与MTT孵育时间.结果:在每孔PBMC为4.0×104(浓度为4.0×105/mL)、药物作用时间48h、MTT 20 μL作用6 h、单波长570 nm处测OD值时,药物对细胞的抑制率较高,试验效果较好.结论:规范化的MTT法体外抗肿瘤药敏试验为临床医生开展和实现个性化化疗提供了可靠的依据. |
| 关 键 词： | 药物筛选试验,抗肿瘤 体外 MTT法 影响因素 |
Influencing Factors of the MTT Based on Tumor Chemosensitivity Assay in Vitro(A Study Report) |
|
| WEI Wen-qing,ZHAO Man-cang,LIU Jing,JIAO Juan,ZHANG Yan,FU Yao,AN Ping. Influencing Factors of the MTT Based on Tumor Chemosensitivity Assay in Vitro(A Study Report)[J]. Medical Journal of Beijing Military Region, 2008, 20(6) | |
| Authors: | WEI Wen-qing ZHAO Man-cang LIU Jing JIAO Juan ZHANG Yan FU Yao AN Ping |
| Affiliation: | WEI Wen-qing,ZHAO Man-cang,LIU Jing,JIAO Juan,ZHANG Yan,FU Yao,AN Ping(Department of Central Laboratory,General Hospital of Beijing Military Area Comm,,Beijing 100700,China) |
| Abstract: | Objective:To establish a standard process of measuring the anti-cancer drug sensitivity with MTT assay by analyzing the influencing factors of the chemosensitivity assay in vitro.Methods:Some influencing factors of MTT assay were found in studying the sensitivity of human peripheral blood mononuclear cells(PBMC) to anti-cancer drugs Oxaliplatin and Paclitaxel and the time of selective culture and MTT incubation time were optimized.Results:It showed that a better result was obtained in the condition with eac... |
| Keywords: | Drug screening assay anti tumor In vitro MTT assay Influencing factors |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |