一氧化氮对大鼠睾丸生殖细胞凋亡作用的研究

目的：探讨一氧化氮（NO）对大鼠睾丸生殖细胞凋亡的影响。方法 采用MTT法观察NO对细胞增殖的抑制作用，透射电镜分析细胞结构变化，末端脱氧核苷酸转移酶（TdT）介导的原位末端标记法（TUNEL）及琼脂糖凝胶电泳检测生殖细胞DNA变化以进一步分析细胞凋亡的特征。结果：MTT法及DNA末端标记法检测表明，NO可抑制细胞增殖，诱导生殖细胞的凋亡，并呈剂量及时间依赖性，NO组细胞凋亡率明显高于对照组（P〈0．01）。透射电镜观察NO处理20h后的生殖细胞，其核染色质凝集、附着于核边缘呈新月形，核固缩、碎裂形成凋亡小体，符合凋亡细胞特征性改变。结论：一氧化氮具有促使生殖细胞凋亡形成的作用，这对不育症的研究有重要的价值。

Study on The Effects of Nitricoxide Induced Apoptosis on The Germ Cell

Objective To study the effect of NO on rat testis germ cell apoptosis. Methods MTT,TdT mediated dUTP nick, labeling (TUNEL) and transmission electro microscopy(TEM) and agarose gel electrophoresis methods was adopted to detect the character of germ cell apoptosis. Results NO can inhibit germ cell growth. Within a certain range of treating time and does, cell were induced apoptosis with a time and does related manner. The apoptosis rate of SNP group was significantly higher than controls (P<0.01). Electron microscopy revealed that germ cell was observed chromatin condensation, marginate in crescents around the nuclear, nuclear pyknosis and formation of apoptotic body after 20 hour SNP-treated.. Conclusion NO could efficiency induce apoptosis of rat testis germ cell, That was very important for infertile study.