微柱凝胶法配血次侧凝集在临床的应用

目的通过对微柱凝胶法配血次侧凝集患者的生化指标及病程分析,探讨其在临床中的价值。方法把27例微柱凝胶法配血仅是次侧凝集而血型鉴定无误、抗筛阴性的患者作为试验组,35例同类型疾病但配血主次侧均相合的患者作为患者对照组;20例健康者作为健康对照组,回顾分析其血中尿素氮(BUN)、肌酐(Cr)、C-反应蛋白(CRP)、B-型脑尿钠肽(BNP)的含量,并进行合并症和病死率的统计。结果 27例次侧凝集的患者中有肺部感染者占了77.8%。试验组BUN、Cr、CRP、BNP阳性率分别是85.2%、55.6%、96.3%、88.9%,与患者对照组及健康对照组比较差异均有统计学意义(P<0.05);试验组BUN、Cr、CRP、BNP含量平均水平分别是(16.55±8.02)mmol/L、(177.7±165.1)μmol/L、(71.54±61.89)mg/L、(864.15±1162.33)pg/mL,除Cr与患者对照组比较差异无统计学意义外,其他指标与患者对照组及健康对照组比较差异均有统计学意义(P<0.05)。试验组与患者对照组病死率分别是66.7%和14.3%,差异有统计学意义(P<0.05)。结论微柱凝胶法配血次侧凝集提示患者病程严重,次侧凝集可以作为判断病程预后的一个指标。

Clinical application of minor agglutination appeared in cross-matching by microtubes-gel test

Objective To find out the clinical value of the minor agglutination appeared in the cross-matching by the microtubes-gel test（MGT） through analyzing the biochemical indicators and the illness course in the patients with minor agglutination. Methods 27 patients with minor agglutination, correct blood grouping in the cross-matc- hing and negative results by MGT in this hospital during 2011 were selected as the test group. Contemporaneous 35 patients with the same illness and the normal results in cross-matching by MGT were selected as the control group and 20 healthy individuals as the healthy control group. Blood urea nitrogen（BUN）,creatinine（Cr）,C reaction protein （CRP） and B brain natriuretic peptide（BNP） contents were retrospectively analyzed. Complications and the death rate were statistically analyzed. Results 27 case-times of minor agglutination showed pulmonary infection,accounting for 77.8%. The positive rates of BUN,Cr,CRP and BNP in the test group were 85.2% ,55.6% ,96.3℃ and 88.90/oo re- spectively. Compared with the patient control group and the healthy control group, the differences in these indexes showed statistical significance（P〈0.05）. The average levels of BUN,Cr, CRP and BNP in the test group were （16.55±8.02）retool/L,（177.7±165.1）μmol/L, （71.54i61.89）mg/L and（864.15±1 162.33） pg/mL. Compared with the patient control group and the healthy control group,the differences except Cr showed statistical significance （P〈0.05）. The death rates in the test group and the patient control group were 66.7% and 14.3% respectively with statistical difference between them（P〈0.05）. Conclusion The minor agglutination appeared in the cross-matching by the microtubes-gel test prompts the severe illness course,which can be used as an index for judging the prognosisof the course.