血液筛查中丙型肝炎病毒检测方法的应用评价

目的比较血液筛查中丙型肝炎病毒的检测方法,并探讨其应用价值。方法收集2011年8～12月血液筛查中酶联免疫吸附试验(ELISA)、丙型肝炎病毒抗体(抗-HCV)阳性标本共41例,使用重组免疫印迹试验进行确证;同时用新创、Ortho两种抗-HCV ELISA试剂以及Architect Anti-HCV Reagent化学发光试剂进行检测,比较三者结果的差异。结果统计学分析显示,3种检测方法的检测结果差异有统计学意义。重组免疫印迹试验检测HCV阳性35例,阴性6例,与Architect化学发光法结果相符,新创ELISA法检测41例全为阳性,Ortho ELISA法检测阳性33例,阴性8例。结论 Ortho试剂对HCV检测的特异性高于新创试剂,化学发光法对HCV检测的特异性高于ELISA法,化学发光法与ELISA法联合检测能提高血液筛查中HCV的阳性检出率,对可疑标本再做重组免疫印迹试验分析。

Application evaluation of methods for detection of hepatitis C virus in blood screening

Objective To compare the detection methods of hepatitis C virus in blood screening and to explore its application value. Methods 4I cases of anti-HCV positive samples detected by enzyme-linked immunosorbent as- say（ELISA） in blood screening during August--December 2011 were collected and performed the confirmatory assay by Western blot. At the same time, two kinds of anti-HCV ELISA reagent provided by Intec and Ortho companies and Architect anti-HCV reagent were adopted for detection. The differences in the results among three methods were compared. Results The statistical analysis showed that the results detected by three methods had statistical differ- ence. In the recombinant immunoblot assay measurement, 35 cases were HCV positive and 6 cases were negative, which conformed to the results by the Architect chemiluminescence immunoassay. In the Intec enzyme immunoassay test,41 cases were all positive. In the Ortho enzyme immunoassay test,33 cases were positive and 8 cases were nega- tive. Conclusion The Ortho reagent is more specific than the Intec reagent for the HCV detection,the chemilumines- cence immunoassay detection is more specific than the enzyme immunoassay. The combined detection of the chemilu- minescence immunoassay and the enzyme immunoassay can improve the detection rate of HCV positive in blood screening. The suspicious sample is further analyzed by the recombinant immunoblot assay.