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复发尖锐湿疣中人乳头瘤病毒相关序列的检测
引用本文:郭华阳,谭骏,纪贤文,钟白玉,叶庆佾. 复发尖锐湿疣中人乳头瘤病毒相关序列的检测[J]. 中国现代医学杂志, 1995, 0(3)
作者姓名:郭华阳  谭骏  纪贤文  钟白玉  叶庆佾
作者单位:第三军医大学分子生物学教研室,第三军医大学附一院皮肤科
摘    要:
用聚合酶链反应及其产物斑点杂交技术,对29例复发尖锐湿疣中人乳头瘤病毒(HPV)相关序列进行检测和分型,结果显示复发尖锐湿疣中:HPV6:65.5%,HPV11:24.1%,HPV16:31.0%,HPV6/11:13.8%,HPV6/16:10.4%,HPV18未检测出来,所用探针以外的型别:3.4%。

关 键 词:尖锐湿疣,复发,人乳头瘤病毒,聚合酶链反应,斑点杂交

DETECTION OF HUMAN PAPILLOMAVIRUS (HPV)RELATED SEQUENCE FROM RECURRING CONDYLOMA ACUMINATA BY GP-PCR AND HYBRIDIZATION
Guo Huayang , Tan Jun ,et al.. DETECTION OF HUMAN PAPILLOMAVIRUS (HPV)RELATED SEQUENCE FROM RECURRING CONDYLOMA ACUMINATA BY GP-PCR AND HYBRIDIZATION[J]. China Journal of Modern Medicine, 1995, 0(3)
Authors:Guo Huayang    Tan Jun   et al.
Affiliation:Guo Huayang , Tan Jun , et al.
Abstract:
cases of biopsy were sequenced by general primer-mediated polymerase chain reaction (GP-PCR) and dot blot hybridization was performed using digoxigenin-labelled specific subtype oligonucleotide probes.Among them , 19 cases were found to harbor HPV 11 DNA(24.1 %) , 9 cases contained sequence homologous to HPV 16 DNA (31. 0%) ,7 cases were found to have two types of HPV DNA.
Keywords:human papillomavirus  condyloma acuminata recurrence  hybridization  PCR
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