| ASE联合微孔板刃天青显色法快速筛选海洋抗菌活性物质 |
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| 引用本文： | 刘平怀,刘洋洋,陈德力,时杰,郝宗娣.ASE联合微孔板刃天青显色法快速筛选海洋抗菌活性物质[J].中国实验方剂学杂志,2010,16(15):176-179. |
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| 作者姓名： | 刘平怀 刘洋洋 陈德力 时杰 郝宗娣 |
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| 作者单位： | 海南大学材料与化工学院,海南优势资源化工材料应用技术教育部重点实验室,海口,570228 |
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| 基金项目： | 国家高技术研究发展计划项目，海南省自然科学基金项目，海南省重点科技计划项目 |
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| 摘 要： | 目的: 建立一种快速筛选和评价抗菌活性物质的方法,并将其应用于海洋抗菌活性物质的筛选。 方法: 在对生物样品的提取过程中引入加速溶剂萃取技术(ASE),同时采用刃天青作为指示剂间接判断不同提取物样品的抗菌特性。 结果: 建立了一种快速筛选抗菌药物的方法,并发现白棘三列海胆、梅氏长海胆、面包海星、秀丽节蛇尾的乙酸乙酯提取物对实验菌生长均具有较强抑制作用。 结论: 该方法克服了常规的纸片琼脂扩散法、试管肉汤稀释法等作为抗菌药物筛选方法的不足,可使数量庞大的生物活性物质中抗菌药物的高通量筛选与评价成为可能。
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| 关 键 词： | 刃天青 抗菌 海洋药物 快速筛选 加速溶剂萃取 |
| 收稿时间： | 2010/7/21 0:00:00 |
Rapid Screening Marine Active Substances by Accelerated Solvent Extraction Combined with Resazurin Assay |
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| LIU Ping-huai,LIU Yang-yang,CHEN De-li,SHI Jie and HAO Zong-di.Rapid Screening Marine Active Substances by Accelerated Solvent Extraction Combined with Resazurin Assay[J].China Journal of Experimental Traditional Medical Formulae,2010,16(15):176-179. |
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| Authors: | LIU Ping-huai LIU Yang-yang CHEN De-li SHI Jie and HAO Zong-di |
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| Institution: | Ministry of Education Key Laboratory of Application Technology of Hainan Superior Resources Chemical Materials,College of Materials and Chemical Engineering, Hainan University, Haikou 570228, China;Ministry of Education Key Laboratory of Application Technology of Hainan Superior Resources Chemical Materials,College of Materials and Chemical Engineering, Hainan University, Haikou 570228, China;Ministry of Education Key Laboratory of Application Technology of Hainan Superior Resources Chemical Materials,College of Materials and Chemical Engineering, Hainan University, Haikou 570228, China;Ministry of Education Key Laboratory of Application Technology of Hainan Superior Resources Chemical Materials,College of Materials and Chemical Engineering, Hainan University, Haikou 570228, China;Ministry of Education Key Laboratory of Application Technology of Hainan Superior Resources Chemical Materials,College of Materials and Chemical Engineering, Hainan University, Haikou 570228, China |
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| Abstract: | |
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| Keywords: | resazurin antibacterial marine drug rapid screening accelerated solvent extraction |
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