土荆皮乙酸B对膀胱癌5637细胞增殖和凋亡的影响

目的:研究二萜类化合物土荆皮乙酸(pseudolaricc acid B)诱导膀胱癌5637细胞凋亡的作用,探讨其作用机制.方法:MTT法测定不同浓度土荆皮乙酸作用后5637细胞的生长情况；流式细胞术检测细胞周期情况；AnnexinV -FITC/PI双染流式细胞术观察土荆皮乙酸作用后细胞的凋亡情况；免疫印迹法检测survivin和caspase-3蛋白表达的变化.结果:土荆皮乙酸能诱导5637细胞凋亡,并明显抑制survivin的表达,同时对caspase-3有上调作用.结论:土荆皮乙酸在体外可抑制5637细胞增殖,诱导其发生凋亡.土荆皮乙酸诱导5637细胞发生凋亡的生物学效应可能与survivin的表达下降,caspase-3的表达上升有关.

Effects of pseudolaric acid B on apoptosis of bladder cancer cell 5637

Objective: To investigate the apoptosis of bladder cancer cell 5637 induced by pseudolaric acid B in vitro and its mechanism. Method: The cell proliferation was detected by MTT assay;the cell cycle was measured by flow cytometry;the cell apoptosis was observed by flow cytometry with Annexin V-FITC/PI double staining; the expressions of survivin protein and caspase-3 protein were detected by Western blot assay. Result: It showed that pseudolaric acid B remarkably induced apoptosis of 5637 cell line. Moreover, pseudolaric acid B suppressed survivin and up-regulated caspase-3 expression. Conclusion: Pseudolaric acid B inhibits the proliferation and induces the apoptosis of 5637 cells. The molecular mechanism of pseudolaric acid B inducing the apoptosis of 5637 cells may be associated with its action of down-regulating the expression of survivin,and up-regulating the expression of caspase-3.