| 高效液相色谱法测定藿补胶囊中淫羊藿苷含量 |
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| 引用本文： | 张辉,王凤林. 高效液相色谱法测定藿补胶囊中淫羊藿苷含量[J]. 中国医药指南, 2011, 9(28): 226-227 |
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| 作者姓名： | 张辉 王凤林 |
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| 作者单位： | 1. 华润三九医药股份有限公司,广东深圳,518029
2. 武警总医院,北京,100039 |
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| 摘 要： | 目的建立藿补胶囊中淫羊藿苷含量高效液相测定方法。方法色谱柱为PhenomenexODS(5μm,250mm×4.6mmID),流动相为乙腈-0.1%磷酸(27∶73),流速1.0mL/min,测定波长为270nm。结果线性范围为0.162～1.62mg(r=0.99999),平均回收率为100.05%,RSD=1.52%(n=6),精密度RSD=1.62%(n=6),重复性RSD=1.94%(n=6)。结论方法简便、稳定、可靠,可用于藿补胶囊中淫羊藿苷含量测定。
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| 关 键 词： | 藿补胶囊 淫羊藿苷 HPLC |
Determination of Icariin in Huobu Capsule by HPLC |
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| ZHANG Hui,WANG Feng-lin. Determination of Icariin in Huobu Capsule by HPLC[J]. Guide of China Medicine, 2011, 9(28): 226-227 |
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| Authors: | ZHANG Hui WANG Feng-lin |
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| Affiliation: | 1 CR Sanjiu Medical ＆ Pharmaceutical Co.,LTD,Shenzhen 518029,China;2 The Armed Police General Hospital,Beijing 100039,China) |
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| Abstract: | Objective To establish a method for determination of icariin in Huobu capsule by HPC.Method Chromatographic conditions was as follows： column of Phenomenex ODS（5μm,250mm×4.6mm ID）,mobile phase consisting of acetonitrile-0.1%H3PO4（27∶73）,flow rate of 1mL min-1,and detection wavelength of 270nm. Results There was a good linear relationship at a range of 0.162-1.62mg（r=0.999 99）,the average recovery was 100.05%（RSD=1.52%）,precision with RSD of 1.62% （n=6）,repetition with RSD of 1.94% （n=6）. Conclusion The method is simple,accurate,reliable,and can be used to determinate icariin in Huobu capsule. |
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| Keywords: | Huobu Capsule Icariin HPLC |
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