RP-HPLC同时测定赤芍3种化学成分

 目的建立了用RP-HPLC对赤芍中芍药苷、芍药内酯苷以及苯甲酸同时定量的方法，并对不同产地赤芍进行考察。方法高效液相色谱法，色谱柱HypersilC₁₈(200 mm×4.6 mm)，流动相：乙腈-水(12∶88)，磷酸调pH=3.0，流速为0.8 mL·min^-1，检测波长为230nm，柱温为室温。用内标法同时测定了赤芍中芍药苷、芍药内酯苷及苯甲酸的含量。结果芍药苷、芍药内酯苷及苯甲酸浓度与峰面积呈良好的线性关系。平均回收率芍药苷为98.6%，RSD为3.0%；芍药内酯苷为98.6%，RSD 为3.1%；苯甲酸为96.3%，RSD为3.2%。结论 本方法测定了17个不同产地、不同批号赤芍样品中3种化学成分的含量，该方法分离度好，快速，简便，重现性好。

Determination of three chemical constituents in Radix Paeoniae Rubra by RP-HPLC simultaneously

OBJECTIVE A reserved phase HPLC method was developed for the determination of paeoniflorin,albiflorin and benzoic acid in red peony root and 17 samples from different habitats were measured. METHODS A Hypersil C₁₈column (200 mm×4.6 mm) was used with the mobile phase of acetonitrile water (12∶88),adjusted pH=3.0 by phosphoric acid.The flow rate was at 0.8mL·min^-1.The detection wavelength was 230 nm at room temperture.17 samples were determined. RESULTS The linear response ranged from 18.24～273.6 μg·mL^-1for paeoniflorin (r=0.999 4,n=7),4.96～74.4 μg·mL^-1for albiflorin (r=0.999 5,n=7) and 2.168～21.68 μg·mL^-1for benzoic acid(r=0.999 8，n=6) respectively.The recoveries were 98.6 % with RSD 3.0 %for paeoniflorin,98.6 % with RSD 3.1 % for albiflorin and 96.3 % with RSD 3.2% for benzoic acid. CONCLUSION The method is quick,simple and repeatable.