生长抑素对大鼠胰腺腺泡细胞内骨架蛋白F-actin分布和细胞外分泌功能的影响

目的研究生长抑素(somatostatin,SS)对八肽胆囊收缩素(cholecystokinin-octapeptide,CCK-8)刺激的大鼠胰腺腺泡细胞内骨架蛋白F-actin分布变化和细胞外分泌功能的影响。方法分离的19只SD大鼠胰腺腺泡细胞,设空白对照组、不同浓度CCK-8组(10-12、10-11、10-10、10-9、10-8mol/L)、SS组(10-7mol/L)、SS+CCK-8组。SS与不同浓度的CCK-8共同孵育胰腺腺泡细胞30min,用淀粉酶、胰蛋白酶试剂盒检测胰腺腺泡细胞淀粉酶分泌率、胰蛋白酶活性,用激光共聚焦显微镜观察细胞内F-actin分布的变化。结果SS稳定了细胞内骨架蛋白结构,明显提高F-actin近细胞顶部/侧基底部的比值(P<0.05);SS与低浓度的CCK-8(10-11~10-10mol/L)共同孵育胰腺腺泡细胞时,CCK-8诱导的细胞淀粉酶分泌明显被抑制(P<0.05),SS与高浓度CCK-8(10-9~10-8mol/L)共同孵育胰腺腺泡细胞时,缓解了高浓度CCK-8诱导的对细胞淀粉酶分泌的抑制效应;SS降低了CCK-8诱导的胰腺腺泡细胞内胰蛋白酶的活性(P<0...

Effect of somatostatin on F-actin distribution and exocrine function in rat pancreatic acinar cells

Objective To investigated the effect of somatostatin(SS)on cholecystokinin-induced alterations of exocrine function and the redistribution of the F-actin in the pancreatic acinar cells.MethodsIn vitro,isolated rat pancreatic acini were divided into 12 groups:normal control group,different concentrations ofcholecystokinin-octapeptide groups(CCK-8)of 10-12,10-11,10-10,10-9 or 10-8 mol/L,SS group(10-7mol/L),SS and different dose CCK-8 groups.The acinar cells were stimulated with different concentrations of CCK...