利用960系统快速鉴定结核分枝杆菌和非结核分枝杆菌的可行性研究

目的　探讨应用BACTEC MGIT960系统的分枝杆菌生长指示管(Mycobacter Growth Indicator Tube,MGIT)加入对硝基苯甲酸(ρ-Nitro benzoic Acid,PNB)区分结核分枝杆菌复合群(MTBC)与非结核分枝杆菌(NTM)的可行性。方法 用含PNB MGIT检测5株标准分枝杆菌菌株的体外抑菌浓度,并对111株临床分离菌株与传统改良罗氏(Lwenstein-Jensen,L-J)PNB鉴定进行比较。结果 以PNB300μg/ml为界,111株临床分离菌株PNB MGIT法与L-J PNB的菌群鉴定相比较符合率为90.09%;鉴定结果报告平均7.72 d,与L-J PNB法比较有显著性差异,检测时间明显缩短(P<0.001)。结论 应用960系统PNB MGIT法鉴定分枝杆菌菌群的方法快速、准确、实用。

Feasibility study on rapid identification of Mycobacterium tuberculosis complex and non-tuberculosis mycobacterium using BACTEC MGIT 960 system

Objective A rapid method for identificating of M.tuberculosis complex(MTBC) and non-tuberculosis mycobacterium(NTM) were studied by adding ρ-Nitro benzoic Acid(PNB) into the Mycobacter Growth Indicator Tube(MGIT) in BACTEC MGIT 960 system.Methods Five strains were tested PNB inhibition concentration in vitro by MGIT.Results To compare with the method of L？wenstein-Jensen(L-J),a criterion of MGIT PNB 300 ug/ml was used to test the 111 clinical strains,the overall agreement rate was 90.09% compared with L-J.The average reporting time of PNB MIGT was 7.72 days,which was shorter and statistically significant difference in compared with the L-J method(P<0.001).Conclusions The PNB MGIT assay can be considered as a rapid,accurate and practical method for the identification of MTB and NTM.