树突状细胞功能与非小细胞肺癌患者生存关系临床研究

目的:研究老年非小细胞肺癌(NSCLC)外周血树突状细胞(DC)功能及与NSCLC进展和生存的关系。方法:选取本院住院NSCLC患者30例作为研究组,30例同年龄段健康人群为对照组,采用流式细胞术(FCM)检测两组人群外周血DC表面分子,同时进行随访,随访结束后对研究组再分为肺癌死亡组和肺癌存活组,并进行统计学处理。结果:研究组患者外周血DC表面分子CD80,CD83,CD86,CD1a平均荧光强度分别为49.46±31.93,41.01±12.72,116.69±13.87和16.83±4.72,对照组分别为95.98±50.27,49.60±14.87,118.11±25.00和19.15±2.32,P值分别为0.000,0.035,0.035,0.008;肺癌死亡组CD80,CD83,CD86,CD1a平均荧光强度分别为44.22±28.90,36.32±8.75,93.17±49.79,14.46±3.11;存活组分别为54.05±34.62,45.93±14.01,98.45±52.20,18.90±4.98,P值分别为0.837,0.035,0.282,0.008。结论:老年NSCLC患者DC表面分子表达较对照组显著降低,说明DC功能抑制;CD80、CD86在存活组与死亡组间无显著差异,CD83、CD1a有显著差异(P<0.05和P<0.01),NSCLC患者CD83、CD1a低表达与患者疾病进展和生存期相关。

Correlation Between Prognosis and Dendritic Cell's Function in Peripheral Blood in Non-Small Cell Lung Cancer Patients

Objective:To investigate the correlation between dendritic cell(DC)'s function in peripheral blood of elderly non-small cell lung cancer(NSCLC)patients and their prognosis.Methods:Thirty elderly NSCLC patients treated in our hospital were enrolled as research group,30normal elderly persons were served as control.The molecules of DC's were assayed by flow cytometry(FCM),and all patients were followed-up after the assay.At the end of follow-up,patients in research group were subdivided into survival group and death group.Results:FCM showed that MFI values of CD80,CD83,CD86,and CD1ain research group were 49.46±31.93,41.01± 12.72,116.69±13.87and 16.83±4.72,respectively,versus 95.98±50.27,49.60±14.87,118.11±25.00and 19.15±2.32in the control,respectively(P=0.000,0.035,0.035,and 0.008,respectively).While MFI values of CD80,CD83,CD86,and CD1awere 44.22±28.90,36.32±8.75,93.17±49.79,and 14.46±3.11respectively in NSCLC death group versus 54.05±34.62,45.93±14.01,98.45±52.20,and 18.90±4.98respectively in NSCLC survival group(P=0.837,0.035,0.282,0.008,respectively).Conclusion:The expression of DCs'molecules in elderly NSCLC patients were markedly lower than that in the control,which revealed the inhibition of DC's function.In the sub-group of NSCLC patients,the expression of CD80and CD86 between survival and death group were not significant(P>0.05),however,the difference of CD83and CD1awere significant(P<0.05and P<0.01),the low expression of CD83and CD1a in elderly NSCLC patients were correlated with the disease progression and survival.