福辛普利对高血压大鼠的心肌保护作用

目的:探讨高血压大鼠心肌组织中胰岛素样生长因子-1(IGF-1)和缝隙连接蛋白43(CX43)的表达,并运用福辛普利进行干预,研究其心肌保护作用。方法:32只健康雄性SD大鼠随机分为空白对照组(n=8)、假手术组(n=8)以及模型组(n=16),模型组以二肾一夹的方法建立,模型建立成功后,随机分为福辛普利治疗组(n=8)和模型对照组(n=8)。治疗组给予福辛普利按照10mg/(kg·d)剂量灌胃,空白对照组、假手术组以及模型对照组给予相同体积的生理盐水灌胃,每日1次。给药8周后,麻醉处死动物,称体重(BW)、心脏(HW)和左心室(含室间隔LVW)重量,计算HW/BW为心脏指数(mg/g),LVW/BW为左心室指数(mg/g),SP免疫组化法检测心室肌组织中IGF-1、缝隙连接蛋白43(CX43)的表达。结果:与空白对照组相比,假手术组血压、心脏指数、左心室指数、IGF-1蛋白表达率、CX43含量变化不明显,模型组则明显升高(P<0.05);与模型对照组相比,福辛普利治疗组上述指标则改善明显(P<0.05)。结论:肾血管性高血压大鼠心室肌组织IGF-1蛋白表达增多,CX43蛋白表达增多,心脏指数及左心室指数增大,运用血管紧张素转换酶抑制剂福辛普利干预后,IGF-1蛋白表达率、CX43蛋白表达减少,心功能有所改善,提示福辛普利可能通过抑制IGF-1表达、降低CX43表达来改善心功能。

Protection of Cardiac Function of Hypertensive Rats by Fosinopril

Objective:To explore the expression of insulin-like growth factor(IGF-1)and connexin 43 (CX43)in the cardiac tissues of two-kidney-one-clip renal vascular hypertensive rats,and to study the protective effect of fosinopril in this process.Methods:Thirty-two healthy male SD rats were randomly divided into three groups,8as normal control group,8as sham operation group, and 16as operation models,which were established by the classic methods of two-kidney-one-clip,and then were sub-divided into hypertension control group and fosinopril control groups for 8in each group.Fosinopril 10mg/kg,once a day,was dissolved in sodium chloride and adminis-trated by gavage for eight weeks in fosinopril group,while the other three groups were administrated the same volume of sodium chloride.Eight weeks later,all rats were anesthetized and killed.The heart and left ventricle were weighed,and the cardiac index and left ventricle index were calculated,SP immunohistochemical staining was used respectively to detect the expression of IGF-1 and CX43.Results:Compared to that of the normal control group respectively,blood pressure,cardiac index,left ventricle index,and the expressions of IGF-1 and protein CX43increased significantly in the hypertension models(P<0.05),and these changes could be alleviated by fos-inopri(P<0.05).Conclusion:In the two-kidney-one-clip renal hypertensive rats,the increment of IGF-1 and CX43 expressions,as well as the increment of cardiac index and left ventricular index,could be alleviated by fosinopril,which suggested that through the ways of decreasing the expression of IGF-1 and CX43,fosinopril could improve the cardiac function.