荧光猝灭法对葫芦巴碱与人血清白蛋白间相互作用的研究

目的:研究葫芦巴碱与人血清白蛋白间的相互作用。方法:在pH 7.4作用液、286nm激发波长条件下采用荧光光谱法来研究不同浓度的葫芦巴碱对人血清白蛋白的荧光猝灭作用。结果:当作用温度为37℃和47℃时,葫芦巴碱与人血清蛋白间的结合常数K分别为749.03L.mol-1和106.29L.mol-1,结合位点数n分别为1.0077和0.7625。结论:葫芦巴碱对人血清白蛋白的荧光发射有一定的猝灭作用,并且温度的升高不利于两者的结合;同时还证明葫芦巴碱与人血清白蛋白分子间的结合力主要是静电作用。

Studying on Interaction between Trigonelline and Humen Serum Albumin by Fluorescence Quenching Method

Objective： To investigate the effect of trigonelline on human serum albumin（HSA）.Methods： The experiment compared the interactions from different concentrations of trigonelline to human serum albumin（HSA） by means of the fluorescence quenching under the conditions of pH 7.4 and 286nm excitation wavelength.Results： When the temperature were 37℃ and 47℃,the binding constant K between trigonelline and HSA were 749.03L·mol-1 and 106.29L·mol-1 with the binding sites（n） value of 1.0077and 0.7625,respectively.Conclusion： The results showed that trigonelline had a ability but not intensively to quench the fluorescence of humen serum albumin（HSA）,and when the reaction temperature is increased,negative effects appear on the binding reaction.The changes of the thermodynamic parameters of binding interaction at 37℃ and 47℃ indicated that the main binding force between trigonelline and humen serum albumin was electrostatic force.