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| 大鼠原位肝移植模型的手术技巧 | |
| 引用本文： | 王振猛,唐乙,宋少华,陆智杰,王全兴,俞卫锋.大鼠原位肝移植模型的手术技巧[J].临床军医杂志,2008,36(4). |
| 作者姓名： | 王振猛 唐乙 宋少华 陆智杰 王全兴 俞卫锋 |
| 作者单位： | 1. 上海东方肝胆外科医院,麻醉科,上海,200438 2. 解放军第二军医大学,基础部免疫学教研室,上海,200433 3. 上海长征医院,移植科,上海,200003 |
| 摘 要： | 目的探讨建立稳定的大鼠原位肝移植模型的外科手术技巧,为研究肝脏缺血再灌注损伤提供技术支持。方法110只雄性SD大鼠随机配对后分别作为供体和受体,通过改良Kamada"二袖套"法施行大鼠原位肝移植。结果改良Kamada法建立大鼠原位肝移植模型共52例,7d生存率88.5%,7例长期存活大于100d。结论大鼠原位肝移植模型的建立需要极度的耐心和娴熟的手术技巧,模型的建立有助于研究肝脏缺血再灌注损伤,移植免疫及器官保存。 |
| 关 键 词： | 原位肝移植 动物模型 大鼠 |
Operation skills for Orthotopic Liver Transplantation in Rats |
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| Wang Zhen-meng,Tang Yi,Song Shao-hua,Lu Zhi-jie,Wang Quan-xing,Yu Wei-feng.Operation skills for Orthotopic Liver Transplantation in Rats[J].Clinical Journal of Medical Officer,2008,36(4). | |
| Authors: | Wang Zhen-meng Tang Yi Song Shao-hua Lu Zhi-jie Wang Quan-xing Yu Wei-feng |
| Institution: | Wang Zhen-meng1,Tang Yi2,Song Shao-hua3,Lu Zhi-jie1,Wang Quan-xing2,Yu Wei-feng1 (1. Department of Anesthesiology,Shanghai Eastern Hepatobiliary Surgery Hospital,Shanghai 200438,China,2. Department of Immunology,College of Basic Medical Science of Second Military Medical University,Shanghai 200433,3. Department of Transplantation,Shanghai Changzheng Hospital,Shanghai 200002,China) |
| Abstract: | Objective To improve the operation technique for orthotopic liver transplantation in rats, and illustrate the hepatic ischemia-reperfusion injury in future study.Methods One hundred and ten male SD rat were randomly paired as donor and receptor and were performed with orthotopic liver transplantation by modified Kamada's two cuffs technique.Results As a result, 88.5% of the 52 rats with orthotopic liver transplantation survived more than 7 days and 7 ones survived longer than 100 days. Conclusion Extreme pa... |
| Keywords: | orthotopic liver transplantation animal model rat |
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