去卵巢大鼠肾脏组织表达FSHR和LHR

 目的 通过研究FSH受体(FSHR)、LH受体(LHR)在大鼠肾脏组织中的表达特性,明确肾脏是否具有和卵巢相似的内分泌功能和变化,为肾脏的生理功能增添新的内容,并为目前临床治疗慢性肾功能衰竭性激素水平减退提供新的思路和方法。方法 制备去卵巢动物模型,采用放免法、RT-PCR半定量法对正常和去卵巢大鼠肾脏组织中FSHR、LHR的表达特性进行研究。结果 肾脏和卵巢中FSH、LH对其受体表达的双相调节是一致的：低剂量FSH、LH提高FSHRmRNA、LHRmRNA水平,而高剂量FSH、LH下调FSHRmRNA、LHRmRNA水平;高剂量的雌激素处理可导致FSHRmRNA水平的显著下降。结论 大鼠肾脏中FSHR和LHR的表达调节特性与卵巢组织一致,肾脏可能具有和卵巢组织相似的内分泌功能和变化,从而参与性激素合成和调节过程。

The expression of FSHR and LHR in ovariectomized rat kidney

Objective By studying the characteristics of FSH receptor (FSHR), LH receptor (LHR) in rat renal tissue, further to prove the similar features and changes in renal and ovary, then to reveal the substantial of " The renal governs reproducion " ,to provide the experimental basis for the theory of traditional Chinese medicine, and to add new contents for the physiological functions of the renal. Methods To prepare the model of ovariectomized rats, then to study the characteristics of FSHR, LHR in normal and ovariectomized rat kidney tissue by radioimmunoassay, RT-PCR semi-quantitative methods. Results The two-phase adjustments of FSH, LH receptor in kidney and ovary is same: low doses of FSH, LH increased the level of the FSHRmRNA, LHRmRNA, while high doses of FSH, LH down the level of the FSHRmRNA, LHRmRNA; the treatment of high-dose estrogen significantly decreased the level of the FSHRmRNA, while the death of the fetal kidney cell suspension effectively increased the level of the E2 in ovariectomized rats, reduced the expression of FSHR mRNA. Conclusion The regulation characteristics of the FSHR,LHR was same in rat kidney and ovarian tissues, kidney and ovarian tissues may have similar functions, to participate in the process of the synthesis and regulation of hormone.