建立人喉鳞癌裸鼠移植模型两种方法的比较

目的:建立人喉鳞癌(Hep-2)裸鼠移植模型,比较两种建模方法成瘤效果。方法:将裸鼠随机分成两组,每组8只,两组分别于皮下接种人喉鳞癌细胞悬液和改良法皮下接种瘤块建立动物模型,观察接种成瘤率、肿块大小、生长速度;免疫组化检测人端粒酶逆转录酶(hTERT)蛋白表达。结果:接种3d后,改良瘤块接种法组裸鼠可见皮下隆起瘤体,接种1周后,皮下接种瘤细胞法和改良瘤块接种法成瘤率分别为75%、87.5%,比较第1周瘤体体积,P<0.05,但成瘤后肿瘤生长方面无明显的差别(比较第3周瘤体体积,P>0.05),两种方法成瘤后的瘤块hTERT蛋白表达阳性率相同。结论:采用改良瘤块接种法成瘤率高,生长速度快,且裸鼠皮下肿瘤较好的继承了人喉鳞癌细胞的重要生物学特点,该法能较好建立了人喉鳞癌裸鼠移植模型,为后续以端粒酶为靶点的体内抗肿瘤研究奠定良好基础。

Comparison of Two Methods of Establishing Transplantation Tumor Model of Nude Mice with Human Larynx Squamous Carcinoma

Objective: Objective: To compare the two methods of establishing a nude mice transplantation tumor model with human larynx squamous carcinoma.Methods: The nude mice were randomly assigned into two groups to receive injection of Hep-2 carcinoma cell suspension and modified injection of tumor masses subcutaneously,to establish the nude mice model with human larynx squamous carcinoma.The successful rate of the tumor transplantation,the size of tumors,and growth speed were observed;the hTERT expression level in...