| 气相色谱法同时检测附桂风湿膏中薄荷脑、冰片和水杨酸甲酯的含量 |
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| 引用本文： | 文庆,聂平,张帆,丁野,郑金凤,李文莉.气相色谱法同时检测附桂风湿膏中薄荷脑、冰片和水杨酸甲酯的含量[J].中南药学,2014(6):585-588. |
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| 作者姓名： | 文庆 聂平 张帆 丁野 郑金凤 李文莉 |
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| 作者单位： | [1]湖南省食品药品检验研究院,长沙410001; [2]中国药科大学,南京210009; [3]湖南中医药大学,长沙410208 |
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| 基金项目： | 国家药典委员会国家药品标准提高行动计划项目（编号：618）. |
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| 摘 要： | 目的建立附桂风湿膏中薄荷脑、冰片、水杨酸甲酯的GC检测方法。方法采用毛细管气相色谱法,色谱柱为EC-WAX毛细管柱(30 m×0.53 mm×0.15μm);柱温(程序升温):初始温度为80℃,保持5 min,10℃·min-1,升至180℃后保持10 min,进样口温度为180℃,检测器温度为230℃;载气为氮气,分流比为10:1,流量:7.5 mL·min-1。结果本法线性关系良好,各精密度RSD均<1.2%,薄荷脑、冰片、水杨酸甲酯的平均加样回收率分别为99.1%、98.8%、101.6%,RSD分别为2.2%、2.9%、2.1%。结论该方法操作简便快速,灵敏度高,准确度好,可作为附桂风湿膏质量控制方法。
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| 关 键 词： | 气相色谱法 附桂风湿膏 薄荷脑 龙脑 水杨酸甲酯 |
Determination of menthol,borneol and methyl salicylate in Fugui Fengshi plaster by GC |
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| Institution: | WEN Qing, NIE Ping, ZHANG Fan, DING Ye, ZHENG Jin-feng, LI Wen-li (1. Hunan Institute for Food and Drug Control, Changsha 410001; 2. China Pharmaceutical university, Nanjing 210009; 3. Department of Pharmaceutical Sciences, Hunan University of Chinese Medicine, Changsha 410208) |
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| Abstract: | Objective To establish a GC method to determine menthol,borneol and methyl salicylate in Fugui Fengshi plaster. Methods GC was performed on an EC-WAX column（30 m×0.53 mm×0.15 μm）. The programming temperature of the column was 80 ℃ keeping 5 min, and then raised to 180 ℃ with the increase rate of 10 ℃·min-1. The injector temperature was 180 ℃, and the FID detector temperature was 230 ℃. The carrier gas was nitrogen, the split ratio was 10 ：1, and the N2 speed was 7.5 mL·min-1. Results There was a good linearity within the experimental concentration. The average recoveries of the 3 constituents were 99.1%, 98.8% and 101.6%, with RSDs of 2.2%, 2.9% and 2.1%, respectively. Conclusion The method is simple, accurate and sensitive. It can be used to identify and evaluate the quality of Fugui Fengshi plaster. |
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| Keywords: | GC Fugui Fengshi plaster menthol borneol methyl salicylate |
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