慢性特发性血小板减少性紫癜患者活化T细胞CD30表达与凋亡关系

本研究检测慢性特发性血小板减少性紫癜(ITP)患者外周血单个核细胞中CD3+数、CD3+/CD30+T细胞比值和活化后T细胞凋亡率及外周血血小板计数,探讨2组间的差异和患者活化T淋巴细胞CD30表达率与凋亡率和血小板计数的相关性。以40例慢性ITP患者为研究对象,24例健康志愿者为正常对照,体外培养刺激T细胞活化后运用流式细胞仪检测外周血单个核细胞悬液中CD3+数、CD3+/CD30+T细胞比值及CD3+T细胞凋亡率,同步检测血小板计数,分析ITP组与对照组各指标间的差异和相关性。结果表明:ITP组外周血CD3+细胞数与对照组无明显差异(p0.05)。ITP组活化T细胞表面CD30表达率明显高于对照组(p0.01)。ITP组活化T细胞凋亡率明显低于对照组(p0.01)。活化后T细胞表面CD30表达与其凋亡率呈负相关(r=-0.786,p0.01)。活化T细胞凋亡率与血小板计数呈正相关(r=0.680,p0.01)。结论:慢性ITP患者活化T细胞CD30的高表达导致了T细胞活化后凋亡受阻,在体内蓄积引起免疫功能紊乱,使血小板减少。

Relationship between Apoptosis and CD30 Expression of Activated T-lymphocyte in Patients with Chronic Idiopathic Thrombocytopenic Purpura

This study was aimed to detect the CD3+ count,ratio of CD3+/ CD30+ T-lymphocytes,the apoptosis rate of activated T-lymphocytes in peripheral blood mononuclear cells and the circulating platelet counts in patients with chronic idiopathic thrombocytopenic purpura(ITP),and to investigate the relationship between them.40 cases of chronic ITP patients were taken as the research group and 24 healthy persons were served as the control group.The CD3 count,ratio of CD3/CD30 T-lymphocytes and the apoptosis rate of ac...