川芎嗪对人肝星状细胞凋亡与基质金属蛋白酶抑制因子-1 mRNA表达的影响

目的:观察川芎嗪(TMP)对人肝星状细胞(HSC)凋亡与基质金属蛋白酶抑制因子-1mRNA(TIMP-1 mRNA)表达的影响。方法:采用人肝星状细胞株LX-2作为理想的肝星状细胞研究模型,LX-2细胞经不同剂量TMP(终浓度为50、100、200、400mg/L)作用48h后,应用流式细胞仪Annexin/PI双染方法检测TMP对LX-2细胞凋亡的影响;应用RT-PCR检测TMP对LX-2细胞TIMP-1 mRNA表达的影响。结果:TMP可显著促进LX-2细胞凋亡,细胞早期凋亡率随TMP浓度增加而提高,与空白对照组相比凋亡率显著升高(P<0.01或P<0.05);同时TMP可显著降低LX-2细胞TIMP-1mRNA的表达,与空白对照组相比差异显著(P<0.01)。结论:促进HSC凋亡,抑制其TIMP-1 mR-NA的表达是TMP抗肝纤维化的部分机制。

Effects of Tetramethylpyrazine on Apoptosis and Tissue Inhibitor of Metal-oproteinase mRNA Expression in Human Hepatic Stellate Cell

Objective:To investigate the effects of tetramethylpyrazine(TMP) on apoptosis and tissue inhibitor of metalloproteinase-1 mRNA(TIMP-1 mRNA)expression in cultured human hepatic stellate cell(HSC)in viro.Methods:LX-2 human hepatic stellate cell line was chosen as the study model of the activated hepatic stellate cell.The apoptosis-promoting effects of TMP were detected by flow cytometry using Annexin/PI-staining and the TIMP-1 mRNA expression was performed by RT-PCR on HSC after 48h culture in different concentrations of TMP.Results:TMP can promote obviously HSC apoptosis and with elevation of TMP,the early apoptosis rate was raised magnificently compared with that of the control group(P<0.01 or P<0.05).At the same time,TMP can decrease magnificently the TIMP-1 mRNA expression compared with that of the control group(P<0.01).Conclusion:The antifibrotic mechanism of TMP might be partly due to promoting apoptosis and inhibiting the TIMP-1 mRNA expression of hepatic stellate cell.