益肾健脾养血通利方对多囊卵巢综合征大鼠模型胰岛素样生长因子-1 mRNA表达的影响

目的观察益肾健脾养血通利方对多囊卵巢综合征（PCOS）大鼠模型胰岛素样生长因子-1（IGF-1）mRNA表达的影响，从而在分子水平初步探讨该方治疗PCOS的可能机制。方法SD雌性大鼠72只，随机分为正常对照组、模型组、克罗米芬组及中药小、中、大剂量组，除正常对照组外，其余各组皮下埋植左旋炔诺孕酮硅胶棒，建立PCOS大鼠模型，应用原位杂交法观察IGF-1 mRNA在大鼠多囊卵巢中的表达。结果模型组IGF-1 mRNA表达明显高于对照组，图像半定量分析显示中药治疗组均能减少IGF-1 mRNA的表达。结论益肾健脾养血通利方具有降低大鼠多裳卵巢中IGF-1 mRNA的表达，改善胰岛素抵抗状况的作用。

Effect of Yishen Jianpi Yangxue Tongli Fang on IGF-1 mRNA Expression of PCOS Rat

Objective To investigate the effects of Yishen Jianpi Yangxue Tongli Fang （YSJP） on IGF-1 mRNA expression in the ovary of PCOS rat, and study its mechanism on the molecular level. Methods Seventy two female SD rats were randomly divided into normal control group, model group, clomifene group and low-, mid-, high-dose Chinese medicine group. The animal model established by capsulae levonorgestreli silasticus was used to observe the IGF-1 mRNA expression in ovary by hybridization in situ. Results IGF-1 mRNA expression of PCOS model group was higher than the normal control group obviously. The semi-quantitative image analysis showed that TCM treatment could decrease IGF-1 mRNA expression. Conclusion Yishen Jianpi Yangxue Tongli Fang could decrease the IGF-1 mRNA expression in ovary and improve the insulin resistance of PCOS rats.