Expression and cellular localization of interleukin-6 mRNA in ovariectomized rats |
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| Authors: | Tu Yihui Du Jingyuan Yang Anli |
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| Affiliation: | (1) Department of Orthopedics, Xiehe Hospital, Tongji Medical University, 430022 Wuhan;(2) Present address: Deptartment of Orthopedics, Shanghai Yangpu Center Hospital, 200090 Shanghai |
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| Abstract: | In order to observe the expression and cellular localization of interleukin-6 (IL-6) mRNA in bone tissue, ovaria of the rats were excised to develop osteoporosis model. The expression of IL-6 mRNA in bone tissues was detected by using dot blot hybridization assay and the cells producing IL6 identified and localized by using in situ hybridization respectively. The results showed that the expression of IL-6 mRNA was significantly increased in the ovariectomized rats as compared with that in normal control rats and strong IL-6 mRNA hybridization signals were detected in lining cells, osteoblasts and osteocytes. It was suggested that loss of ovarian function induced in vivo osteoblast lineage increased IL-6 mRNA expression. IL-6 might play important roles in the development of bone loss following ovariectomy. |
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| Keywords: | interleukin-6 ovariectomy dot blot hybridization in situ hybridization |
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