| HPLC法测定乌饭树叶中乌饭树苷含量 |
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| 引用本文： | 赵建萍,吴云,牛忻,张庸雪,徐幸琦,刘江云,郝丽莉.HPLC法测定乌饭树叶中乌饭树苷含量[J].上海中医药杂志,2017,51(10):100-102. |
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| 作者姓名： | 赵建萍 吴云 牛忻 张庸雪 徐幸琦 刘江云 郝丽莉 |
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| 作者单位： | 上海城建职业学院健康安全系,上海,201415,苏州大学医学部药学院中药系,江苏苏州,215123;南通卫生高等职业技术学校药学及医学技术系,江苏南通,226000,苏州大学医学部药学院中药系,江苏苏州,215123,南通卫生高等职业技术学校药学及医学技术系,江苏南通,226000,南通卫生高等职业技术学校药学及医学技术系,江苏南通,226000,南通卫生高等职业技术学校药学及医学技术系,江苏南通,226000,南通卫生高等职业技术学校药学及医学技术系,江苏南通,226000 |
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| 基金项目： | 国家自然科学基金资助项目(U1603124) |
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| 摘 要： | 目的：建立乌饭树叶中主要活性成分乌饭树苷的含量测定方法。 方法：利用染色活性跟踪法,分离鉴定有效成分；采用高效液相色谱法测定其含量,色谱柱为Cosmosil-5C18 - PAQ(4.6 mm × 250 mm,5 μm),流动相为0.3%乙酸溶液乙腈梯度洗脱,流速1.0 ml/min,检测波长320 nm。 结果：乌饭树苷为乌饭树叶主要有效成分,其色谱分析的分离度良好,在0.052 08 ～ 0.520 8 g/L范围内线性良好,r值为0.999 8,加样回收率为98.61%。 结论：该有效成分测定方法灵敏度高,结果准确、简便,可为乌饭树叶的质量控制方法提供参考。
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| 关 键 词： | 乌饭树 乌饭树苷 HPLC 质量控制 |
| 收稿时间： | 2017/6/1 0:00:00 |
Content determination of vaccinoside in leaves of Vaccinium bracteatum Thunb. by HPLC |
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| ZHAO Jian-ping,WU Yun,NIU Xin,ZHANG Yong-xue,XU Xing-qi,LIU Jiang-yun and HAO Li-li.Content determination of vaccinoside in leaves of Vaccinium bracteatum Thunb. by HPLC[J].Shanghai Journal of Traditional Chinese Medicine,2017,51(10):100-102. |
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| Authors: | ZHAO Jian-ping WU Yun NIU Xin ZHANG Yong-xue XU Xing-qi LIU Jiang-yun and HAO Li-li |
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| Institution: | Health and Social Care Institute,Shanghai Urban Construction Vocational College,Shanghai,201415,Department of Chinese Medicine,College of Pharmaceutical Sciences,School of Medicine,Soochow University,Suzhou,215123;Department of Medical Technology, Nantong Higher Health Vocational and Technical School, Nantong, Jiangsu, 226000,Department of Chinese Medicine,College of Pharmaceutical Sciences,School of Medicine,Soochow University,Suzhou,215123,Department of Medical Technology, Nantong Higher Health Vocational and Technical School, Nantong, Jiangsu, 226000,Department of Medical Technology, Nantong Higher Health Vocational and Technical School, Nantong, Jiangsu, 226000,Department of Medical Technology, Nantong Higher Health Vocational and Technical School, Nantong, Jiangsu, 226000 and Department of Medical Technology, Nantong Higher Health Vocational and Technical School, Nantong, Jiangsu, 226000 |
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