| 胶质母细胞瘤11 号-染色体杂合性丢失的研究 |
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| 引用本文： | 胡杰,江澄川,吴浩强,彭颂先,唐婉君. 胶质母细胞瘤11 号-染色体杂合性丢失的研究[J]. 中国临床神经科学, 2002, 10(1): 25-27 |
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| 作者姓名： | 胡杰 江澄川 吴浩强 彭颂先 唐婉君 |
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| 作者单位： | 1. 复旦大学附属华山医院神经外科,200040
2. 香港中文大学病理解剖及细胞学系 |
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| 摘 要： | 目的 :寻找胶质母细胞瘤 (GBM) 11号染色体上可能存在肿瘤抑制基因的杂合性丢失 (LOH)区域。方法 :应用聚合酶链反应方法 ,采用荧光标记的引物和ABIPRISMTM3 77型DNA序列自动分析仪 ,分析GBM 11号染色体上 18个微卫星多态性标记的LOH。结果 :在 47 6%GBM 11号染色体上观察到LOH ,11p和 11q的LOH率分别为 42 9%和 2 3 8%。 11q上所有位点的LOH率都较低 (<3 0 % ) ,在 11p上的下列区域检测到较高的LOH率 :11p 15 5上的D11S40 46(3 3 3 % )位点、11p12 p13上的D11S90 5 (4 1 2 % ) D11S93 5(3 7 5 % )区域。结论 :在 11p 15 5上的D11S40 46位点、11p12 p13上的D11S90 5 D11S93 5区域可能存在多个与GBM相关的肿瘤抑制基因
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| 关 键 词： | 杂合性丢失 胶质母细胞瘤 肿瘤抑制基因 |
| 文章编号： | 1008-0678(2002)01-025-03 |
| 修稿时间： | 2001-03-19 |
A Study of Loss of Heterozygosity on Chromosome 11 in Glioblastoma |
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| Abstract: | Aim:To locate the deletion areas probably harboring tumor suppressor genes(TSGs) on chromosome 11 of GBM.Methods:Polymerase chain reaction based microsatellite polymorphism analyses was performed to detect LOH on chromosome 11,fluorescence labeled primers and ABI PRISM TM 377 DNA Sequencer were applied.Results:LOH on chromosome 11 was observed in 47 6% informative cases of GBM.The LOH frequencies of 11p and 11 q are 42 9% and 23 8% respectively.The LOH frequencies of all loci on chromosome 11 q are low(<30).High LOH frequencies were observed in the following chromosomal regions on 11p:from loci D11S905 (41 2%)to D11S935 (37 5%) on 11p12 p13 and at loci D11S4046(33 3%)on 11p15 5.Conclusion:The chromosomal regions from loci D11S905 to D11S935 on 11p12 p13 and at loci D11S4046 on 11p15 5 may harbor multiple TSGs associated with GBM. |
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| Keywords: | loss of heterozygosity(LOH) glioblastoma(GBM) tumor suppressor gene(TSG) |
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