| 核苷磷酸化酶的产酶条件优化的研究 |
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| 引用本文： | 闫蓬勃,邱蔚然,丁庆豹.核苷磷酸化酶的产酶条件优化的研究[J].药物生物技术,2001,8(2):83-85. |
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| 作者姓名： | 闫蓬勃 邱蔚然 丁庆豹 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海 200237 |
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| 摘 要： | 以鸟苷和5－氟尿嘧啶为底物，乙酰短杆菌菌体为酶源酶法合成5－氟尿苷，采用酵线浸膏培养基（酵母浸膏4．0％，葡萄糖0．4％，NaCl0.3%,pH7.0)，较高的溶氧量，同时添加0．6％的豆油，培养12h，产酶量可提高30％。
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| 关 键 词： | 乙酰短杆菌 核苷磷酸化酶 5－氟尿苷 产酶条件 |
| 文章编号： | 1005-8915(2001)02-083-03 |
Study on Optimal Conditions of NPase |
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| Yan Pengbo,Qiu Weiran,Ding Qingbao.Study on Optimal Conditions of NPase[J].Pharmaceutical Biotechnology,2001,8(2):83-85. |
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| Authors: | Yan Pengbo Qiu Weiran Ding Qingbao |
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| Abstract: | 5-Fluorouride was enzymatically synthes ized from guanosine and 5-fluor ouracil by brevibacterium acetylicum. The suitable culture medium contained 40g yeast extract, 4g glucose, 3g NaCl in 1 liter while pH was adjusted to 7.0 with NaOH. After brevibacterium acetylicum was cultured for 12 h in the conditi on of the higher dissolve oxygen and 0.6% soybean oil supplied , the amount of enzym produced can be increased by 30%. |
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| Keywords: | Brevibacterium acetylicum Nucleoside phosphorylase 5-Fluorouridine |
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