Biochemical and X-ray micro-computed tomographic analyses of critical size bone defects grafted with autogenous bone and mercerized bacterial cellulose membranes salified with alendronate期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Biochemical and X-ray micro-computed tomographic analyses of critical size bone defects grafted with autogenous bone and mercerized bacterial cellulose membranes salified with alendronate

Institution:	1. School of Health Sciences, Positivo University, 5300 Professor Pedro Viriato Parigot de Souza Street, Curitiba, 81280-330, PR, Brazil;2. Department of Periodontics and Oral Hygiene, School of Dentistry, The University of Texas Health Science Center at Houston, 7500 Cambridge St, Houston, 77054, TX, USA;3. Department of Medical Anatomical Sciences, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 615 E 3rd St Pomona, 91766, CA, USA;4. Master Graduation on Urban and Industrial Environments, Polytechnic Center, Federal University of Paraná, 100 Cel. Francisco H. dos Santos Avenue, Curitiba, 81530-000, PR, Brazil;5. Department of Periodontics, IOA Boutique Curitiba, 6823 Sete de Setembro Avenue, Curitiba, 80240-001, PR, Brazil;6. Department of Stomatology, School of Dentistry, Federal University of Parana, 632 Prefeito Lothario Meissner Avenue, Curitiba, 80210-170, PR, Brazil;1. Department of Zoology, Guru Ghasidas Vishwavidyalaya, Bilaspur, India;2. Department of Biochemistry, Late Atal Bihari Vajpayee Memorial Medical College Rajnandgaon (C.G.), India;3. Department of Public Health, Nellore Municipal Corporation, Nellore, India;4. Department of Immunopathology, Institute of Lungs Biology and Disease, Comprehensive Pneumology Center, Helmholtz Zentrum, Neuherberg, Munich, 85764, Germany;1. Department of Periodontology, Torabinejad Dental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran;2. Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran;1. Division of Physiology, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan;2. Section of Primary Dental Education, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan;3. Division of Oral and Maxillofacial Radiology, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan;1. Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;2. Gerontology, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;3. Oral Functional Anatomy, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;4. Periodontology and Endodontology, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;5. Orthodontics, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;6. Oral Functional Prosthodontics, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;7. Northern Army Medical Unit, Camp Makomanai, Japan Ground Self-Defense Forces, Sapporo, Japan;8. Shandong Provincial Key Laboratory of Oral Biomedicine, The School of Stomatology, Shandong University, Jinan, China;1. Department of Orthodontics, Matsumoto Dental University, Shiojiri, Nagano, Japan;2. Division of Oral and Maxillofacial Biology, Institute for Oral Science, Matsumoto Dental University, Shiojiri, Nagano, Japan;3. Department of Oral and Maxillofacial Biology, Graduate School of Oral Medicine, Matsumoto Dental University, Shiojiri, Nagano, Japan;4. Department of Prosthodontics, Matsumoto Dental University, Shiojiri, Nagano, Japan;5. Department of Oral Physiology, Graduate School of Dentistry, Osaka University, Suita, Japan

Abstract:	ObjectivesThis study aimed to evaluate the repair of critical-sized bone defects grafted with autogenous bone and mercerized bacterial cellulose membranes (BCm) salified with alendronate (ALN).MethodsForty-eight male Wistar rats underwent surgery to create a 5 mm-diameter bone defect in the calvarium. The removed bone was particularized, regrafted into the defect, and covered by a BCm according to the group: control group (CG), simply mercerized BCm; group 1 (G1), negatively charged BCm (BCm-CM^-) salified with ALN; and group 2 (G2), positively charged BCm (BCm-DEAE⁺) salified with ALN. Serum samples were collected preoperatively and before euthanasia to analyze osteoprotegerin (OPG), parathyroid hormone (PTH), sclerostin (SOST), and fibroblast growth factor 23 (FGF23) levels. The animals were euthanized after 15 or 60 d. Calvaria were analyzed using quantitative microtomography (μCT).ResultsThere was an increased level of PTH in the CG compared to the G2 group, at day 60 (p = 0.019). When analyzing the same group over time, G1 presented an increased FGF23 level on days 15 and 60 (p < 0.05). CG presented an increase in PTH (p = 0.037) at day 60. The μCT analysis detected increased trabecular separation on day 15 in G2 compared to G1 (p = 0.040).ConclusionsSalification of ionized BCm with ALN had no direct effect on bone repair; however, BCm-CM^- increased the levels of FGF23 over time. BCm-DEAE⁺ decreased PTH levels compared to mercerized BCm. BCm-CM-salified with ALN-induced superior bone quality, with respect to trabecular separation, compared to BCm-DEAE⁺.

Keywords:	Alendronate Skull Bone Bone regeneration ALN"} {"#name":"keyword" "$":{"id":"kwrd0035"} "$$":[{"#name":"text" "_":"sodium alendronate ANOVA"} {"#name":"keyword" "$":{"id":"kwrd0045"} "$$":[{"#name":"text" "_":"analysis of variance BCm"} {"#name":"keyword" "$":{"id":"kwrd0055"} "$$":[{"#name":"text" "_":"bacterial cellulose membrane FGF23"} {"#name":"keyword" "$":{"id":"kwrd0065"} "$$":[{"#name":"text" "_":"fibroblast growth factor 23 OPG"} {"#name":"keyword" "$":{"id":"kwrd0075"} "$$":[{"#name":"text" "_":"osteoprotegerin PTH"} {"#name":"keyword" "$":{"id":"kwrd0085"} "$$":[{"#name":"text" "_":"parathyroid hormone SD"} {"#name":"keyword" "$":{"id":"kwrd0095"} "$$":[{"#name":"text" "_":"standard deviation SDS"} {"#name":"keyword" "$":{"id":"kwrd0105"} "$$":[{"#name":"text" "_":"sodium dodecyl sulfate SOST"} {"#name":"keyword" "$":{"id":"kwrd0115"} "$$":[{"#name":"text" "_":"sclerostin μCT"} {"#name":"keyword" "$":{"id":"kwrd0125"} "$$":[{"#name":"text" "_":"micro-computed tomography
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