Ｅｚｒｉｎ和ＡＫＴ２在大肠癌组织中的表达及其临床意义

【摘　要】　目的：通过免疫组化检测Ｅｚｒｉｎ和ＡＫＴ２在大肠癌和大肠正常组织的表达水平，结合临床病理因素，探讨Ｅｚｒｉｎ和ＡＫＴ２的表达水平与大肠癌发生发展、转移及预后的关系，为寻找大肠癌基因治疗新的干预靶点提供实验和理论依据。方法：用免疫组织化学ＥｎＶｉｓｉｏｎ法检测９５例大肠癌组织和癌旁正常组织４０例中的Ｅｚｒｉｎ和ＡＫＴ２的表达水平，并对９５例大肠癌按照有无淋巴结转移进行分组比较，分析其与大肠癌临床病理参数的关系。结果：４０例大肠癌旁正常组织中，Ｅｚｒｉｎ蛋白表达阳性４例，占１０.０％，ＡＫＴ２表达阳性７例，占１７.５％，而在９５例大肠癌组织中，Ｅｚｒｉｎ蛋白表达阳性７４例，占７７.９％，ＡＫＴ２表达阳性５５例，占５７.９％。Ｅｚｒｉｎ和ＡＫＴ２表达与淋巴结转移有关，在淋巴结转移组Ｅｚｒｉｎ和ＡＫＴ２阳性率明显高于无淋巴结转移组（Ｐ＜０.０５）。ＡＫＴ２阳性表达组中Ｅｚｒｉｎ蛋白表达阳性率明显高于ＡＫＴ２阴性表达组中的表达（Ｐ＜０.０５）。Ｅｚｒｉｎ和ＡＫＴ２共表达的４９例患者，淋巴结转移率为６１.２％，而Ｅｚｒｉｎ和ＡＫＴ２均不表达的１６例患者无１例淋巴结转移。结论：Ｅｚｒｉｎ和ＡＫＴ２在大肠癌组织中高表达，与大肠癌转移密切相关，并可作为临床判断转移及预后等生物学行为的重要参考指标。Ｅｚｒｉｎ和ＡＫＴ２在大肠癌组织中表达成正相关，可联合检测判断大肠癌的恶性程度和进行预后评估。

Expression of Ezrin and AKT2 and its clinical significance in human colorectal carcinoma

Objective：This study aims to test Ezrin and AKT2 expressions in colorectal carcinoma, adjacent normal colorectal mucosa and combine with some clinical pathological factors to discuss the relationship between expressions of Ezrin and AKT2 and the progress, metastasis and prognosis of colorectal carcinoma. Methods ： Immunohistological En Vision staining was used to detect the expressions of Ezrin and AKT2 in 95 eases of human colorectal carcinoma and 40 cases of adjacent normal colorectal mucosa and the different expressions of Ezfin and AKT2 among lymph nodes metastatic and non-metastatic human colorectal carcinoma,including 54 metastatic cases and 4l non-metastatic cases. The relationship of Ezrin and AKT2 expressions with clinicopathological factors in colorectal carcinoma was also analyzed. Results：In adjacent normal colorectal mucosa, the expression of Ezrin protein was found in 4 cases among 40 cases（ positive rate 10% ）. The expression of AKT2 was found in 7 cases among 40 cases（ positive rate 17.5% ）. In colorectal carcinoma,the expression of Ezrin protein was found in 74 cases among 95 cases （positive rate 77. 9% ）, the expression of AKT2 was found in 55 cases among 95 cases（ positive rate 57. 9% ）, and the expressions of Ezrin and AKT2 had close relation to lymph node metastasis. The positive rates of Ezrin and AKT2 expressions in lymph node metastasis cases were obviously higher than those without lymph node metastasis. The expression of Ezrin protein in AKT2 positive cases was obviously higher than those of AKT2 negative cases. The lymph node metastatic rate was 61.2% in 49 patients with both positive expression of Ezrin and AKT2,while no lymph node metastasis were found in the 16 cases with both negative. Conclusion ：The increase in expression of Ezrin and AKT2 was closely correlated to metastasis in human colorectal carcinoma. It might be served as an important factor for determining tumor biological behavior and evaluating prognosis and metastasis. Positive correlations were observed between the expression of Ezrin and AKT2 in human colorectal carcinoma. Combinational examination can evaluate prognosis and metastasis.