HA／TCP双相陶瓷与人成骨细胞生物相容性的体外实验研究

体外复合培养条件下，通过观察人成骨细胞与HA／TCP的复合生长及监测材料对细胞生理功能的影响来评价材料的生物相容性。研究发现，复合培养过程中成骨细胞首先贴附于材料的表面，进而攀附于材料边缘切刻处及材料表层微孔的边缘，以后逐渐长入孔中。最后，材料几乎为细胞所覆盖。复合培养的成骨细胞与正常培养的成骨细胞一样，具有分泌大量胶原纤维、表现强烈碱性磷酸酶活性和形成矿化胞外基质等成骨表型。细胞能够与材料很好地复合生长、材料对细胞生理功能又无明显的影响表明HA／TCP与人成骨细胞具有良好的生物相容性。

Biocompatibility of HA/TCP Biphasic Ceramics with Co-cultured Human Osteoblasts in vitro

The biocompatibility of HA/TCP ceramic was evaluated by investigation of attachment and growth of osteoblasts on biomaterial, as well as monitoring the effects of biomaterial on expression of functional phenotypes of co cultured osteoblasts in vitro. When co cultured with HA/TCP ceramics, osteoblasts firstly attached to the surface of HA/TCP disk, then attached to notches and grew into the micropores of biomaterial during further culture period. At last, the ceramics were almost packed with osteoblasts. Additionally, osteoblasts co cultured with HA/TCP were similar to osteoblasts cultured under normal condition in osteoblastic phenotypes; the secreted lots of collagen type I, possess strong activity of Alkaline Phosphatase and mineralizd extracellular matrix. The fact that osteoblasts could grow well on HA/TCP ceramics and the biomaterial did not affect their physiological function suggest that HA/TCP ceramic is biocompatible with human osteoblasts.