| 梅毒螺旋体重组蛋白Tp0463的表达与抗原性鉴定 |
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| 引用本文： | 刘小军,吴移谋,曾铁兵,赵飞骏,余坚,牛淑会,姚玲.梅毒螺旋体重组蛋白Tp0463的表达与抗原性鉴定[J].中国现代医药杂志,2011,13(9):4-6. |
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| 作者姓名： | 刘小军 吴移谋 曾铁兵 赵飞骏 余坚 牛淑会 姚玲 |
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| 作者单位： | 1. 南华大学附属第一医院血液科
2. 南华大学医学院病原生物学研究所,衡阳,421001 |
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| 基金项目： | 湖南省科技厅重点项目，湖南省自然科学基金重点项目 |
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| 摘 要： | 目的表达梅毒螺旋体(Tp)重组蛋白Tp0463(rTp0463)并鉴定其抗原性,为进一步探讨其在梅毒血清学诊断和免疫保护中的作用奠定基础。方法 PCR扩增Tp0463基因,构建原核表达重组体pET-28a(+)/Tp0463,转化宿主菌诱导表达重组蛋白,Ni-NTA亲和层析法纯化蛋白,Westernblot鉴定表达产物的抗原性。结果成功构建了原核表达重组体pET-28a(+)/Tp0463,经诱导高效表达了一分子量大约为16KDa的重组蛋白,以包涵体表达形式为主,纯化蛋白的纯度>90%;Westernblot结果显示纯化蛋白能被梅毒患者混合血清特异性识别。结论高效表达了rTp0463,该重组抗原有良好的抗原性。
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| 关 键 词： | 梅毒螺旋体 梅毒 Tp0463 重组抗原 |
Expression and antigenicity idenfication of recombinant protein Tp0463 of Treponema pallidum |
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| Liu Xiaojun,Wu Yimou,Zeng Tiebing,et al..Expression and antigenicity idenfication of recombinant protein Tp0463 of Treponema pallidum[J].Modern Medicine Journal of China,2011,13(9):4-6. |
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| Authors: | Liu Xiaojun Wu Yimou Zeng Tiebing |
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| Institution: | Liu Xiaojun,Wu Yimou,Zeng Tiebing,et al.The Institute of Pathogenic Biology of University of South China,Hengyang 421001 |
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| Abstract: | Objective To express and identify antigenicity of recombinant protein Tp0463(rTp0463) of Treponema pallidum(Tp),and to provide a basis for further investigation of its significance in syphilis serodiagnosis and immunoprotection.Methods Tp0463 gene was amplified by PCR.The prokaryotic expression recombinant pET-28a(+)/Tp0463 was constructed and transformed into E.coli BL21 for induced expression of recombinant proteins which were purified with Ni-NTA affinity chromatography.Western blot was performed to iden... |
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| Keywords: | Treponema pallidum Syphilis Tp0463 Recombinant antigen |
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