苓桂术甘汤对免疫功能低下模型小鼠淋巴细胞活性的影响

目的:观察苓桂术甘汤对免疫功能低下模型小鼠免疫功能的影响.方法:近交系昆明种小鼠以环磷酰胺(Cy,80 mg/kg,ip,qd×1)诱导免疫功能低下,用苓桂术甘汤(42.90,21.45,4.29 g/kg)连续灌胃(ig)给药7 d,分别采用免疫溶血法、四氮唑盐比色法(MTT)和小鼠胸腺细胞氚标胸腺嘧啶核苷(3H-TdR)掺入法测定血清溶血素、NK细胞及IL-2活性.结果:苓桂术甘汤能明显促进Cy所致免疫功能低下模型小鼠血清溶血素生成,增强NK细胞及IL-2活性,与模型组比较,差异有显著性(P＜0.05或P＜0.01).结论:苓桂术甘汤对Cy所致免疫功能低下模型小鼠三类淋巴细胞的免疫活性均具有明显的激活作用.

Effect of Linggui Zhugan Decoction on Lymphocyte Activities in Immunosuppressed Mice Induced by Cyclophosphamide

Objective:To study the effect of Linggui Zhugan Decoction on lymphocyte activities in immunosuppressed mice. Method: The immunosuppressed mice were induced by intraperitoneal injection of cyclophosphamide 80 mg/kg once daily. Linggui Zhugan Decoctions (42.90, 21.45, 4.29 g/kg) were administrated by intragastric injection to immunosuppressed mice once daily for 7 days. Then the content of serum hemolysin, theactivities of natural killer cell (NK) and interleukin 2 (IL 2) were measured by immunohemolysis, MTT assay and thymus cells intermingled 3 H TdR respectively. Result: Linggui Zhugan Decoction could obviously enhance the quantity of hemolysin in serum, and also evidently promote the activities of NK and IL 2 in immunosuppressed mice. Conclusion: Linggui Zhugan Decoction could markedly promote the immunological function of immunosuppressed mice induced by cyclophosphamide.