bcl-2基因表达在genistein抑制裸鼠肝癌生长中的作用

目的：探讨bcl-2基因表达变化在genistein治疗裸鼠移植人肝癌中的作用。方法:以裸鼠移植人肝癌为对照, 对照组腹腔注入含0.04%DMSO RPMI-1640培养基0.05 L/kg，genistein治疗组腹腔注入genistein 1 mg kg-1·d-1，3周后观察肝癌增长情况，并应用同位素试剂盒检测肝癌组织IP3 含量,RT-PCR分析癌组织bcl-2 mRNA表达，Western blotting 分析肝癌组织Bcl-2蛋白表达。结果:治疗组肝癌体积和重量均显著低于对照组［体积:（42.7±27.8)mm3 vs (52.3±26.5)mm3;重量:(42.7±27.8)mg vs (91.3±31.4)mg］，IP3含量显著低于对照组［(13.4±1.4)nmol/g protein vs （35.3±6.6）nmol/g protein］，bcl-2 mRNA表达显著低于对照组［RI（灰度与面积之积的相对强度）0.48±0.02 vs 0.56±0.15］, P<0.01，Bcl-2蛋白表达显著低于对照组［RI（灰度与面积之积的相对强度）1.69±0.52 vs 1.37±0.48］。结论:Genistein能减少IP3 生成,下调肝癌组织bcl-2基因表达,抑制裸鼠移植肝癌的生长。

Role of bcl-2 gene expression in inhabition of hepatocellular carcinoma by genistein in nude mice

AIM:To probe into the role of 1, 4, 5 - trisphosphate inositol (IP3) and bcl-2 gene expression in inhibiting hepatocellular carcinoma of nude mice by genistein. METHODS:Animals with hepatocellular carcinoma were treated with genistein 1 mg·kg-1·d-1 (ip) for 3 weeks. The volume and weight of tumaor were measured. IP3, bcl-2 mRNA, Bcl-2 protein were assayed by IP3-［3H］ Birtrak assay, RT-PCR, Western blotting, respectively. RESULTS:The tumor volume and weight of animals treated with genistein were lower than those in control (42.7mm3±27.8mm3 vs 52.3mm3±26.5mm3, 42.7mg±27.8 mg vs 91.3mg±31.4 mg). IP3 content was lower than that in control ［(13.4±1.4)nmol/g protein vs (35.3±6.6)nmol/g protein］. bcl-2 mRNA expression was lower in group treated with genistein than that in control (RI which was the gray degree multiply area of bcl-2 / the gray degree multiply area of β-actin 0.48±0.02 vs 0.56±0.15). Bcl-2 protein expression was lower in group treated with genistein than that in control (RI 1.69±0.52 vs 1.37±0.48). CONCLUSION:Genistein inhibits growth of transplanted hepatocellular carcinoma in nude mouse liver by reducing IP3 production and down-regulating bcl-2 gene expression.