| 抗人喉癌双功能抗体制备及对Hep-2细胞株杀伤作用的研究 |
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| 引用本文： | 杨桂琴,孙立群,管国芳,辛丁. 抗人喉癌双功能抗体制备及对Hep-2细胞株杀伤作用的研究[J]. 中国耳鼻咽喉头颈外科, 2000, 7(3): 164-166 |
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| 作者姓名： | 杨桂琴 孙立群 管国芳 辛丁 |
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| 作者单位： | 130041,长春白求恩医科大学附属第二临床学院耳鼻咽喉-头颈外科;130041,长春白求恩医科大学附属第二临床学院耳鼻咽喉-头颈外科;130041,长春白求恩医科大学附属第二临床学院耳鼻咽喉-头颈外科;130041,长春白求恩医科大学附属第二临床学院耳鼻咽喉-头颈外科 |
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| 基金项目： | 国家自然科学基金!39470747 |
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| 摘 要： | 目的：为了制备高效的,特异的抗人喉癌／抗蓖麻毒素A双功能单克隆抗体,观察其对Hep－2细胞株杀伤作用。方法：选用BALB／C鼠,分别行蓖麻毒素A和人喉鳞状细胞癌腹腔免疫后,提取抗体,经与骨髓瘤细胞融合,制备成抗人喉癌／抗蓖麻毒素A双特异性单克隆抗体。结果：该双功能抗体与喉癌组织的特异性结合率为87％,对Hep－2细胞半数抑制浓度比游离环磷酰胺强40倍。结论：采用二次杂交瘤技术制备的双功能抗体优于化学偶联法,抗体效价具有均匀性和理想的信噪比,抗体易于标准化。
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| 关 键 词： | 喉肿瘤(Laryngeal neoplasms) 癌(Carcinoma) 抗体 肿瘤(Antibodies noplasm) |
| 修稿时间： | 1999-11-22 |
Preparation of human laryngeal cancer bispecific antibody and its killing effect to Hep-2 cell line |
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| Yang Guiqin,Sun Liqun,Guan Guofang,Xin Ding. Preparation of human laryngeal cancer bispecific antibody and its killing effect to Hep-2 cell line[J]. Chinese Archives of Otolaryngology-Head and Neck Surgery, 2000, 7(3): 164-166 |
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| Authors: | Yang Guiqin Sun Liqun Guan Guofang Xin Ding |
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| Abstract: | To investigate the preparation of a human laryngeal cancerbispecific antibody. Methods: The second set hybrid tumor technology was applied to prepare an anti-human laryngeal cancer and anti-castor toxin A bispecific monoclonal antibody. Results: The specific conjugation rate between bispecific monoclonal antibody and laryngeal cancer tissue was 87%. The half inhibition concentration to Hep-2 cell was 40 times stronger than that of free cyclophosphamide. Conclusion:The second set hybrid tumor technology to prepare the bispecific antibody had a homogeneous titer,an idealsignal-to-noise ratio and was easy to be standardized. |
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