HPLC法测定狼疮肾病患者口服吗替麦考酚酯后的血药浓度

目的:建立测定狼疮肾病患者口服吗替麦考酚酯后血药浓度的方法。方法:狼疮肾病患者血样处理后采用高效液相色谱法测定吗替麦考酚酯体内代谢物霉酚酸。色谱柱为SGE Protecol C18,流动相为甲醇-乙腈-0.01%磷酸(23∶37∶40,V/V/V),检测波长为216nm,流速为1.0ml/min,进样量为20μl,柱温为30℃。结果:霉酚酸血药浓度在0.125～10mg/L范围内线性关系良好(r=0.9999);方法回收率为98.67%～104.31%;萃取回收率为83.43%～97.54%,内标(尼美舒利)萃取回收率为79.35%;日内、日间RSD分别为2.6%～7.7%、2.3%～8.5%。结论:本法简单、准确,可用于狼疮肾病患者服用吗替麦考酚酯后霉酚酸的血药浓度检测。

Determination of the Blood Concentration of Mycophenolate Mofetil in Lupus Nephritis Patients by HPLC

OBJECTIVE： To determine the blood concentration of mycophenolate mofetil （MMF） in the lupus nephritis （LN） pa tients. METHODS： HPLC was used for determining MMF metabolite mycophenolic acid （MPA） after blood sample of LN patients trated. SGE Protecol C18 column was used with mobile phase consisted of methanol-acetonitrile0.01% phosphoric acid （23：37：40, V/V/V） at the flow rate of 1.0 ml/min. The detection wavelength was set at 216 nm, and injection volume was 20 . The column temperature was 30℃ RESULTS： The linear range of MPA was 0.125-10 mg/L （r=0.999 9） with method recovery of 98.67%-104.31% and extraction recovery of 83.43%-97.54%. The extraction recovery of internal standard （nimesulide） was 79.35%. RSDs of inter-day and intra-day were 2.6 %-7.7 % and 2.3 %-8.5 %, respectively. CONCLUSIONS： The method is simple and accurate, and can be used for blood concentra- tions monitoring of MPA in LN patients after taking MMF.