震荡对血小板聚集功能检测的影响

目的 研究震荡对血小板聚集功能检测的影响.方法 选取2016年8月到2016年11月就诊于大连医科大学附属第一医院临床确定诊断为冠状动脉粥样硬化性心脏病的患者40例,采用PL-12血小板功能分析仪检测震荡前后血小板聚集功能,诱导剂分别是花生四烯酸(arachidonic acid,AA)和二磷酸腺苷(adenosine disphosphate, ADP),分析血小板最大聚集率(maximum aggregation rate,MAR)和平均聚集率(average aggregation rate,AAR).结果 以AA为诱导剂组,震荡前全血标本的MAR为31.93±19.86,震荡后全血标本MAR 28.83±17.31,两者比较差异无统计学意义(t=1.128,P>0.05);震荡前全血标本的AAR为33.27±19.78,震荡后全血标本AAR 29.95±15.98,两者比较差异无统计学意义(t=1.320,P>0.05).以ADP为诱导剂组,震荡前全血标本的MAR为54.48±15.31,震荡后全血标本MAR 45.61±17.20,两者比较差异有统计学意义(t=3.668,P<0.05);震荡前全血标本的AAR为55.34±18.1,震荡后全血标本AAR 49.24±17.45,两者比较差异有统计学意义(t=2.682,P<0.05).结论 MAR和AAR是临床判断抗血小板聚集药物疗效的重要指标,震荡会对全血标本血小板聚集功能中MAR和AAR产生影响,故在日常检验工作中操作人员应避免不当的震荡,以免造成错误的检测结果 ,影响临床对患者血小板功能的评估.

Effect of shaking on platelet aggregation function test

Objective To study the effect of shaking on platelet aggregation function test.Methods Totally, 40 patients with coronary atherosclerotic heart disease diagnosed in the First Affiliated Hospital of Dalian Medical University from August 2016 to November 2016 were selected as subjects in the study.PL-12 platelet function analyzer was used to detect platelet aggregation function.MAR and AAR were detected before and after shaking.Inducers used in this study were AA and ADP.Results In AA inducer group, MAR of blood samples were 31.93±19.86 before shaking and 28.83±17.31 after shaking, respectively;there was no significant difference between the two groups (t=1.128,P>0.05).AAR of blood samples were 33.27±19.78 before shaking and 29.95±15.98 after shaking, respectively;there was no significant difference between the two groups (t=1.320,P>0.05).In ADP inducer group, MAR of blood samples were 54.48±15.31 before shaking and 45.61±17.20 after shaking, respectively;the difference was statistically significant (t=3.668,P<0.05).AAR of blood samples were 55.34±18.1 before shaking and 49.24±17.45 after shaking, respectively;the difference was statistically significant (t=2.682,P<0.05).Conclusion MAR and AAR are important indicators to evaluate platelet function and drug efficacy for clinical physicians.Shaking will affect the results of MAR and AAR.For laboratory technologists, inappropriate shaking may lead to false results and should be avoided.