脂联素基因单核苷酸多态性与急性心肌梗死的相关性研究

目的研究脂联素基因单核苷酸多态性（SNP）与急性心肌梗死（AMI）的相关关系。方法采用聚合酶链反应-限制性片段长度多态法（PCR-RFLP）检测78例AMI患者和84例健康正常对照者脂联素基因外显子2 SNP＋45和内含子3 SNP＋276基因型,分别比较2组基因型分布和等位基因频率。结果 AMI组中脂联素基因SNP＋276为GT基因型者明显高于对照组（P〈0.05）,而等位基因频率未见显著性差异;脂联素基因SNP＋45基因型分布和等位基因频率2组间未见显著性差异。AMI组中脂联素基因SNP＋276为GG＋GT基因型者血糖浓度显著高于TT基因型者（P〈0.05）;AMI组脂联素基因SNP＋45为GG基因型者胆固醇浓度高于TT和TG基因型者（P〈0.05）。结论脂联素基因SNP＋276与AMI有相关关系,GT基因型者具有急性心肌梗死高易感性;脂联素基因外显子SNP＋45和内含子SNP＋276基因多态性可能分别与AMI高危因素胆固醇和血糖浓度有关。

Associations between single nucleotide polymorphisms of adiponectin gene and acute myocardial infarction

Objective To investigate the associations of single nucleotide polymorphisms(SNPs)+45 or+276 of adiponectin gene with acute myocardial infarction(AMI).Methods Eighty-four healthy volunteers and seventy-eight AMI patients undergoing coronary angiograms were enrolled.Genotypings for the two SNPs of adiponectin gene were conducted by Taqman polymerase chain reaction(PCR).Results Association of SNP+276 with AMI was observed(P=0.042).But there was no association of SNP+45 with AMI(P=0.301).Also significant assoc...