北京分离株金黄色葡萄球菌基因型分析

目的对2000-2005年北京分离株金黄色葡萄球菌进行基因分型研究，了解其型别构成特征。方法分别对48株耐甲氧西林金黄色葡萄球菌（methicillin-resistant S．aureus，MRSA）和3株甲氧西林敏感金黄色葡萄球菌（methicillin-sensitive S．aureus，MSSA）进行spa基因序列分析及脉冲场凝胶电泳（pulsed field gel electrophoresis，PFGE）分析，并与多位点序列分析（multi locus sequencing typing，MLST）进行比较。结果在MRSA中，t030和t037为主要spa型别，分别占47．92％（23／48）和37．50％（18／48）；t002只在2005年菌株中出现，占14．58％（7／48）。3株MSSA型别分别为t377和t304。PFGE按带型差异及相似度分析，将所有菌株分为5组11型。结论t030和t037是MRSA临床分离株中的spa优势型别，A组和D组为PFGE优势型别。合理使用MLST、PFGE和spa分型方法对我国MRSA防治具有重要意义。

Genotyping of Staphylococcus aureus strains isolated from Beijing

Objective To analyze the genotype of Staphylococcus aureus strains isolated from the Beijing population during the years from 2000 to 2005 and to obtain a primary knowledge of the use of different genotyping methods. Methods Forty-eight methicillin-resistant S. aureus （MRSA） strains and three methicillin-sensitive S. aureus （MSSA） strains were analyzed on the basis of spa polymorphisms and by pulsed field gel electrophoresis（PFGE）, and they were compared with the results of multi locus sequence typing （MLST）. Results t030 and t037 were the most prevalent strain types, accounting for 47.92 % （23/48） and 37.50 % （18/48） of all MRSA strains, respectively, while t002 was only isolated 2005 and accounted for 14.58% （7/48）. t377 and t304 were the spa types of the three MSSA strains. The PFGE patterns of the 51 strains belonged to five groups and eleven types. Conclusion t030 and t037 are the most prevalent spa types among clinical MRSA strains and are different types from those of MSSA strains. Group A and D are the most prevalent PFGE types. Rational combination of spa typing, MLST and PFGE will be of great significance in MRSA control and prevention.