miR-452在肾透明细胞癌中的异常表达研究

目的检测miR-452在肾透明细胞癌组织中的表达差异,探讨miR-452在肾癌发生过程中的作用。方法提取20例肾透明细胞癌及其相应癌旁组织的总RNAs,通过逆转录(RT)方法获得cDNA后,采用实时定量PCR(RT-qPCR)技术检测组织中miR-452的表达差异,并分析其与肾癌患者临床病理特征的关系。结果与相应的癌旁组织相比,miR-452在肾透明细胞癌组织中显著高表达,癌组织较癌旁组织升高约4.69倍,但其异常表达与肾癌患者临床病理特征并无显著相关性。结论 miR-452在肾透明细胞癌组织中明显高表达,其可能作为重要的致癌基因参与了肾癌的发生与发展过程,可能成为潜在的肾癌标志物,为肾癌的早期诊断及治疗提供新靶点。

Research on Abnormal Expression of miR-452 in Clear Cell Renal Cell Carcinoma

Objective To investigate the differential expression level of miR-452 in tissue samples of clear cell renal cell carcinoma. Furthermore, we attempt to explore the biological effects of miR-452 in the process of renal cell carcinoma. Methods The total RNAs were isolated from human clear cell renal cell carcinoma and matched adjacent normal tissues of 20 patients with clear cell renal cell carcinoma. RT was performed firstly to get the cDNAs, then qPCR was used to detect the expression level of miR-452 in samples above and then the relationship was analysed between the altered miR-452 and the clinical pathologic characteristics of patients with clear cell renal cell carcinoma, Results Compared with the matched adjacent tissues,miR-452 was significantly high expression in clear cell renal cell carcinoma tissues. MiR-452 was up-regulated 4.69 folds on average than adjacent tissues. However,the expression level of miR-452 had no correlation with the clinical pathologic characteristics of patients with renal cell carcinoma. Conclusion MiR-452 was significantly high expression in tissues of clear cell renal cell carcinoma. This suggested miR-452 might be as a key oncogene closely related with the occurrence and deyelopment of renal cell carcinoma and maybe served as a potential tumor marker of renal cell carcinoma,and provided a novel target for the early diagnosis and therapy of renal cell carcinoma.