青蒿琥酯对脂多糖诱导的大鼠肾小球系膜细胞凋亡及Bcl-2基因表达的影响

目的：观察青蒿琥酯（Art）对脂多糖（LPS）诱导的大鼠肾小球系膜细胞（glomerular mesangial cells,GMC）凋亡及Bcl-2基因表达的影响,并探讨其可能的作用机制。方法：用不同浓度的青蒿琥酯刺激脂多糖诱导增殖的大鼠肾小球系膜细胞,HE染色观察凋亡细胞形态,DAPI荧光染色观察细胞核凋亡情况,用流式细胞仪检测细胞凋亡率,免疫细胞化学结构和计算机图文分析系统检测Bcl-2的表达。结果：青蒿琥酯对系膜细胞凋亡有明显诱导作用,呈一定剂量依赖性;并能明显地抑制脂多糖诱导上调的Bcl-2基因的表达,且高浓度组的抑制作用强于低浓度组。结论：青蒿琥酯能够剂量依赖性的诱导大鼠系膜细胞凋亡,其机制可能与调控Bcl-2的表达有关。

Effect of Artesunate on Apoptosis of Rat Glomerular Mesangial Cells and Expression of Gene Bcl-2 Induced by Lipopolysaccharide

Objective.To investigate the effect of artesunate on apoptosis of rat glomerular mesangial cells（GMC） induced by lipopolysaccharide（LPS）, and to explore the possible mechanism, Methods： Mesangial cells were incubated with different concentrations of artesunate induced by lipopolysaccharide, Morphology of apoptosis was discriminated by HE staining;karyon apoptosis was examined by fluorescence staining of DAPI; cell apoptosis rate was detected, by flow cytometry, The expressions of Bcl - 2 were detected by immunocytochemistry and computed video text analysis system, Results： Artesunate can obviously induce apoptosis of GMC in a dose - dependent manner to some extent. The expressions of Bcl - 2 were decreased after incubating with Artesunate, which was more significant in high concentration group than that in low concentration group. Conclusion：Artesunate induces apoptosis of GMC in a dose- dependent manner,while its molecular mechanism may be that Artesunate can down - regulate the expression of Bcl - 2 gene.