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Influence of donor age,post‐mortem time and cold storage on metabolic profile of human cornea |
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| Authors: | Tomasz Kryczka Jacek P. Szaflik Jerzy Szaflik Anna Midelfart |
| Affiliation: | 1. Faculty of Medicine, Department of Neuroscience, Norwegian University of Science and Technology, Trondheim, Norway;2. Department of Medical Biology, Medical University of Warsaw, Warsaw, Poland;3. Department of Ophthalmology, Medical University of Warsaw, Warsaw, Poland;4. Warsaw Eye Bank, Warsaw, Poland;5. Department of Ophthalmology, University Hospital, Trondheim, Norway |
| Abstract: | Purpose: Limited knowledge exists about the influence of donor age and death‐to‐preservation interval (DPI) on the metabolic properties of the cornea. The aim of this study is to investigate the relationship between both factors and metabolite content of the cornea. Methods: Corneas from 15 human donors (age: 41–78 years) were obtained within 16 hrs post‐mortem and kept in cold storage for 8 days. The metabolic profiles of the samples were investigated using high‐resolution, magic angle spinning 1H nuclear magnetic resonance spectroscopy before and after 8 days of preservation. Results: Twenty‐two metabolites were detected and assigned in the corneal spectra. The significant metabolic differences before and after hypothermic storage were revealed between younger and older donors. DPI‐related significant differences revealed before preservation of the corneas were not displayed after 8 days of cold storage. Conclusions: Age of donor as well as post‐mortem time influences the biochemical properties of the cornea. Cold storage decreases the metabolite differences between the tissues collected at different post‐mortem time. |
| Keywords: | cold storage death‐to‐preservation interval donor age human cornea metabolic profile NMR spectroscopy post‐mortem time |