HPLC法测定小蓟饮片中蒙花苷含量

目的研究小蓟中蒙花苷简便快速的含量测定方法,为评价药材及饮片质量提供方便。方法采用高效液相色谱法,测定3种产地小蓟中蒙花苷的含量。色谱条件：Lichrospher C18色谱柱（4.6mm×200mm,5μm）,柱温30℃,甲醇-水（45∶55）为流动相,流速1.0mL.min-1,检测波长326nm。结果小蓟中蒙花苷在0.09981～0.79848μg范围内具有良好的线性关系良好（r=0.9998）,平均回收率为99.89%,RSD=2.84%。结论经过系统的方法学考察,该方法具有简便、专属、稳定、可重复的特点,可用于小蓟中蒙花苷的含量测定。

Determination of linarin in herb of herba Cirsii by HPLC

OBJECTIVE HPLC was used to establish a method for determination of linarin in herb of herba Cirsii.METHOD The chromatographic conditions were：Lichrospher C18 column（4.6mm×200mm,5μm）,methoanol-water（45∶55）as mobile phase,flow rate being 1.0mL·min-1,column temperature at 30℃,detection wavelength at 326nm.RESULT The liner range of linarin was 0.09981～0.79848μg（r=0.9998）,the average recovery was 99.89% and RSD was 2.84%.CONCLUSION The method is proved to be simple,rapid and accurate and may be used to determine linarin in herb of herba Cirsii.