Pomegranate peel extract polyphenols induced apoptosis in human hepatoma cells by mitochondrial pathway |
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| Affiliation: | 1. Department of Medicine, Division of Hematology-Oncology, VA Greater Los Angeles Healthcare System, Los Angeles, CA, 90073, USA;2. Technical Institute of Physics and Chemistry, Chinese Academy of Science, Beijing, 100190, China;3. Department of Urology, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095, USA;4. Department of Medicine, Division of Hematology-Oncology, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095, USA;1. Cancer Biology Laboratory & DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB), Department of Biosciences & Bioengineering, Indian Institute of Technology Guwahati, Assam 781039, India;2. Stem Cell and Cancer Biology Laboratory, School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Perth, WA, 6009, Australia;3. Department for Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh City 700000, Viet Nam;4. Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, Viet Nam;6. Cancer Science Institute of Singapore, National University of Singapore, Singapore;7. Medical Science Cluster, Yong Loo Lin School of Medicine, National University of Singapore, Singapore;8. Curtin Medical School, Faculty of Health Sciences, Curtin University, Perth, WA, Australia;9. National University Cancer Institute, National University Health System, Singapore;1. Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia;2. Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia;1. CBQF – Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Dr. António Bernardino Almeida, 4200-072 Porto, Portugal;2. IPOA Research Group (UMH-1 and REVIV-Generalitat Valenciana), AgroFood Technology Department, Escuela Politécnica Superior de Orihuela, Miguel Hernández University, Crta, Beniel km. 3, 2, E-03312 Orihuela, Spain;1. Department of Community Nutrition, Faculty of Nutrition, Tabriz University of Medical Sciences, Attar street, 5166614711 Tabriz, Islamic Republic of Iran;2. Faculty of medicine, Tabriz University of Medical Sciences, Tabriz, Islamic Republic of Iran;3. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Islamic Republic of Iran;4. Road Traffic Injury Research Center, Tabriz University of Medical Sciences, Attar street, 5166614711, Tabriz, Islamic Republic of Iran;5. Department of Biochemistry and Diet Therapy, Tabriz University of Medical Sciences, Attar street, 5166614711, Tabriz, Islamic Republic of Iran |
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| Abstract: | This study was aimed to investigate the influence of pomegranate peel polyphenols (PPPs) on the proliferation and apoptosis of HepG2 cells (a kind of human hepatoma cells) and the related mechanism. The inverted fluorescence microscope and the flow cytometer (FCM) were used to test the changes of the cellular morphology, cell cycle, apoptosis, reactive oxygen species (ROS) and mitochondrial transmembrane potential (Δψm). The kit was used to measure the activities of caspase-3/9, and Western Blot was used to detect the expressions of apoptosis-associated proteins including p53, Bcl-2/Bax, Cyt-c and PARP. The results showed that the cells cycle of HepG2 arrested at the S-phase by PPPs and the amount of the early apoptotic cells and ROS level were increased obviously, the level of Cyt-c and the activity of Caspase-3/9 markedly were also increased by PPPs, as well as the ratio of Bax/Bcl-2 and the protein expressions of P53. It was concluded that PPPs could inhibit the growth of HepG2 cells by blocking the cell cycle and inducing the mitochondrial apoptotic pathway in a dose-dependent manner. |
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| Keywords: | Pomegranate peel polyphenols Human HepG2 hepatoma cell Antiproliferative Apoptosis |
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