颈椎间盘退变的形态学观察和生物力学研究

目的:观察长时间异常应力环境下兔颈椎间盘的组织形态和生物力学性能变化,为防治颈椎病的研究提供实验依据.方法:选取30只家兔,随机分为对照组、模型组,造模家兔颈椎处于低头屈曲45°位异常应力环境下5小时/次·天.动物处死后,光镜和电镜下观察颈椎间盘组织形态学变化;功能节段生物力学性能测试.比较各组间存在的差异.结果:对照组颈椎间盘组织形态及生物力学性能无明显变化;模型组颈椎间盘发生软骨细胞变性、坏死,髓核皱缩,软骨终板钙化、断裂等组织形态学改变;椎间盘压缩、扭转生物力学性能下降,并随着异常应力作用时间的延长而表现更为显著.结论:长时间处于异常应力环境下能使兔颈椎间盘组织形态和生物力学性能均发生明显退行性改变.

Histopathological and Biomechanical Research of Cervical Intervertebral Disc Degeneration

Objective: To provide experimental data for neck disease prevention by characterizing the changes of cervical intervretebral disc's histopathology an d biomechanics associated with disc degeneration through keeping the rabbits cer vical spine into 45-degree flexion for 5 hours once a day. Methods: 30 Japanese white rabbit were randomly divided into control groups and model groups. Rabbits of the model group were kept at 45-degree flexion for 5 hours once a day. After sacrificed, the histopathological changes were observed under light microscopy and transmission electronic microscopy; Boing the regular biomechanical capabil ity on the function spinal unit was tested; The difference between two groups we re created. Results: Rabbits in the control group showed no histopathology and b iomechanical changes on cervical disc; In the model group, the pathological chan ges of the intervertebral disc include: spit of the annulus fibrosis, dehydratio n of nuclear puiposis, calcification? ossification and separation of the cartila ge endplate; Biomechanical test showed an significant decrease of resist-compres sion character of the intervertebral disc. The longer the flexion time, the more significant the results were. Conclusion: The abnormal stress condition for lon g time conduce to recessive chang on the rabbit cervical intervertebral disc's h istopathology and biomechanics capability.